Liu Xiaoli, Fu Yun, Li Xinxin, Li Yuan, Liu Jizhuang, Hu Yang, Xueting Hong, Ding Xunhuan, Sun Tao
College of Life Science; Huaibei Normal University; Huaibei; 235000; China.
Zootaxa. 2025 Mar 10;5601(3):476-490. doi: 10.11646/zootaxa.5601.3.4.
Kingdonella qinghaiensis Zheng, 1990 a species belonging to the genus Kingdonella Uvarov, 1933, within the family Acrididae in the order Orthoptera, is mainly distributed in Qinghai province, China. In this study, we determined, assembled and annotated the mitochondrial genome of Kingdonella qinghaiensis. The mitogenome is 15,597 bp in length and contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and a control region (D-loop). The entire mitogenome exhibits a strong A/T bias, with an A+T content of 75.4%. All 13 PCGs had the typical start codon of ATN (9 ATGs, 2 ATCs and 2 ATTs) and TAA was the most frequent stop codon in Kingdonella qinghaiensis PCGs, except TAGs for ND3 and ND1 genes. A total of 3,730 codons are present in the mitogenomic PCGs of Kingdonella qinghaiensis. Among these, UUA (9.65%) is the most frequent codon for leucine (L), followed by AUU (9.3%) for isoleucine (I), and UUU (8.12%) for phenylalanine (F). The Ka/Ks ratios of the 13 PCGs in Kingdonella qinghaiensis mitogenome ranged from 0.1436 to 0.9107 (0<Ka/Ks<1), and ND4L had the highest ratio while COX1 gene had the lowest value. The nucleotides diversity (Pi) analysis of the 13 PCGs from 46 species in Acrididae implied that ND2 gene contained the highest variability site (0.27862). While ND5, ND4 and ND1 had comparatively low nucleotide diversities. The phylogenetic tree based on the nucleotide sequences of the 13 PCGs from 46 different species (including 2 outgroups) supported the monophyly of Acrididae and indicated two stable clades in Acrididae. The phylogenetic analyses represented the main topology as follows: ((((Catantopinae+Calliptaminae) +Cyrtacanthacridinae) + ((Spathosterninae+Oxyinae) + Melanoplinae)) + (((Oedipodinae+Acridinae) + Gomphocerinae) +Coptacrinae)). Species from Melanoplinae in the phylogenetic tree confirmed that Kingdonella qinghaiensis had the closer taxonomy relationship with Kingdonella bicollina, another member in the same genus.
青海孔蜢（Kingdonella qinghaiensis），郑，1990年，属于孔蜢属（Kingdonella），乌瓦罗夫，1933年，隶属于直翅目蝗科，主要分布于中国青海省。在本研究中，我们测定、组装并注释了青海孔蜢的线粒体基因组。该线粒体基因组长度为15597 bp，包含13个蛋白质编码基因（PCGs）、22个tRNA基因、2个rRNA基因和一个控制区（D-loop）。整个线粒体基因组表现出强烈的A/T偏好，A+T含量为75.4%。所有13个PCGs都有典型的起始密码子ATN（9个ATG、2个ATC和2个ATT），TAA是青海孔蜢PCGs中最常见的终止密码子，但ND3和ND1基因的终止密码子为TAG。青海孔蜢线粒体基因组的PCGs中共有3730个密码子。其中，UUA（9.65%）是亮氨酸（L）最常见的密码子，其次是异亮氨酸（I）的AUU（9.3%）和苯丙氨酸（F）的UUU（8.12%）。青海孔蜢线粒体基因组中13个PCGs的Ka/Ks比值范围为0.1436至0.9107（0<Ka/Ks<1），ND4L的比值最高，而COX1基因的值最低。对蝗科46个物种的13个PCGs进行核苷酸多样性（Pi）分析表明，ND2基因的变异位点最高（0.27862）。而ND5、ND4和ND1的核苷酸多样性相对较低。基于46个不同物种（包括2个外类群）的13个PCGs核苷酸序列构建的系统发育树支持蝗科的单系性，并表明蝗科中有两个稳定的分支。系统发育分析的主要拓扑结构如下：（(((斑腿蝗亚科+皱腹蝗亚科)+瘤锥蝗亚科)+((佛蝗亚科+尖翅蝗亚科)+黑蝗亚科))+(((笨蝗亚科+蝗亚科)+槌角蝗亚科)+癞蝗亚科))。系统发育树中黑蝗亚科的物种证实，青海孔蜢与同属的另一个成员双脊孔蜢（Kingdonella bicollina）具有更近的分类学关系。
