Huang Da, Haddad Lucy, Rahman Fahmida, Sapelkin Andrei, Palma Matteo
Department of Chemistry, Queen Mary University of London, London, UK.
Department of Physics and Astronomy, Queen Mary University of London, London, UK.
Methods Mol Biol. 2025;2901:67-87. doi: 10.1007/978-1-0716-4394-5_6.
Super-resolution optical imaging techniques rely on validation standards. In this regard, it is of paramount importance to develop consistent and reliable well-defined reference samples. In this study, we employ a DNA origami scaffold to engineer a multicolor quantum dot hybrid nanostructure, and evaluate it through a recently proposed quantum dot-based spectral separation technique. Our findings underscore the utility of multivalent DNA structures, which serve as a robust and precise scaffold for the nanoscale placement of quantum dots. The employed spectral resolution method offers a straightforward and rapid means of imaging acquisition, compatible with standard confocal or fluorescence microscopes possessing spectral signal separation capabilities and a single excitation laser wavelength. This combined methodology represents a promising avenue for advancing super-resolution optical imaging techniques.
超分辨率光学成像技术依赖于验证标准。在这方面，开发一致且可靠的明确定义的参考样本至关重要。在本研究中，我们采用DNA折纸支架来构建多色量子点混合纳米结构，并通过最近提出的基于量子点的光谱分离技术对其进行评估。我们的研究结果强调了多价DNA结构的实用性，它为量子点的纳米级定位提供了一个强大而精确的支架。所采用的光谱分辨率方法提供了一种直接且快速的成像采集手段，与具有光谱信号分离能力和单一激发激光波长的标准共聚焦或荧光显微镜兼容。这种组合方法代表了推进超分辨率光学成像技术的一条有前景的途径。
