Harini Priya M, Bhat Sham S, Sundeep Hegde K
Department of Pedodontics and Preventive Dentistry, Yenepoya Dental College Hospital, Mangalore, Karnataka State, India.
J Clin Pediatr Dent. 2010 Fall;35(1):23-9. doi: 10.17796/jcpd.35.1.u57p4500360g2752.
Since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use ofantibacterial irrigating agents and root canal filling materials. Recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals ofprimary teeth. The study determined the antibacterial effectiveness of four root canal filling materials namely Calcium hydroxide, Zinc oxide eugenol, Vitapex and Metapex against microbial specimens obtained directly from necrotic root canals of primary teeth.
Microbial specimens were collected using sterile paper points, from 15 primary maxillary and mandibular posterior teeth of randomly selected children in the age group of 4-10 years with infected non vital primary teeth, requiring pulpectomy procedure. The microbial specimens collected were subjected to microbiological analysis and the antimicrobial potential of root canal filling materials were tested using Agar diffusion technique.
were statistically analyzed using one-way ANOVA. Facultative/Aerobic organisms were isolated in all the cases, anaerobic organisms were isolated in 80% of the cases, and Candida albicans was isolated in 1 case. ZOE showed superior inhibitory activity against most of the organisms isolated followed by Vitapex, Calcium hydroxide and Metapex in descending order.
Our data may be useful as a guide for relative antimicrobial effectiveness or non-effectiveness of the materials employed. In vivo studies are required to state the specific antimicrobial activity and merits and demerits of any of the test filling material.
由于乳牙根管解剖结构高度多变，实际不可能完全清创乳牙根管，因此牙髓治疗的成功部分取决于抗菌冲洗剂和根管充填材料的使用。近期文献表明，厌氧菌在乳牙坏死根管中的细菌中占大多数。本研究测定了四种根管充填材料，即氢氧化钙、氧化锌丁香酚、Vitapex和Metapex对直接从乳牙坏死根管中获取的微生物标本的抗菌效果。
使用无菌纸尖从随机选取的4至10岁患有感染性非活髓乳牙且需要进行牙髓摘除术的儿童的15颗上颌和下颌乳后牙中收集微生物标本。对收集的微生物标本进行微生物学分析，并使用琼脂扩散技术测试根管充填材料的抗菌潜力。
采用单因素方差分析进行统计学分析。所有病例均分离出兼性/需氧菌，80%的病例分离出厌氧菌，1例分离出白色念珠菌。氧化锌丁香酚对大多数分离出的微生物显示出较强的抑制活性，其次是Vitapex、氢氧化钙和Metapex，活性依次递减。
我们的数据可作为所使用材料相对抗菌有效性或无效性的指导。需要进行体内研究以阐明任何一种测试充填材料的具体抗菌活性及其优缺点。
