双激光流式细胞术：通过单个透镜聚焦时的焦距补偿。

Dual laser flow cytometry: focal length compensation when focussing through a single lens.

作者信息

Fellner-Feldegg H

出版信息

Cytometry. 1985 Jul;6(4):286-9. doi: 10.1002/cyto.990060403.

Abstract

Dual laser operation of flow cytometers, using a single focussing lens for both beams, requires compensating for chromatic aberration of the lens. By using a prefocussing lens at a fixed position in one of the laser beams, complete focal length compensation is obtained without any loss in system performance.

摘要

流式细胞仪的双激光操作，即对两束光都使用单个聚焦透镜，需要补偿透镜的色差。通过在其中一束激光束中的固定位置使用预聚焦透镜，可以实现完全的焦距补偿，且系统性能不会有任何损失。