Ogino M
Endocrinol Jpn. 1985 Feb;32(1):175-80. doi: 10.1507/endocrj1954.32.175.
In the present study, we used an explant culture system of the human decidual tissues involving a new sampling method for investigating the productivity of immunoreactive prolactin (IR-PRL) for a 5 day period in labored and nonlabored deliveries. The maximal release of IR-PRL in the incubation medium for each 24 hour interval was achieved from the 2nd to the 3rd day of culture in both groups, which was 145.2 +/- 14.0 ng/ml/10 mg w.w. (mean +/- s.e.; n = 7) in the labor group and 101.5 +/- 16.3 ng/ml/10 mg w.w. (mean +/- s.e.; n = 4) in the nonlabor group. The release of IR-PRL in both groups was not significantly different for the first 3 days. However, the amount of IR-PRL released in the nonlabor group was 50 to 70% of that of the labor group. The tissue content of IR-PRL in both groups ranged between 3.0 and 5.0 ng/ml/10 mg w.w. From these results, it was concluded that 1) our explant culture system for the human decidual tissues produced considerably more IR-PRL than those previously reported and 2) productivity of IR-PRL was lower in nonlabored delivery than in labored delivery and 3) since the tissue content of IR-PRL for each 24 hour interval was very small, it should be strongly emphasized that the production and release of IR-PRL takes place simultaneously in the human decidual tissues.
在本研究中，我们使用了人蜕膜组织的外植体培养系统，该系统涉及一种新的采样方法，用于研究足月分娩和未足月分娩情况下免疫反应性催乳素（IR-PRL）在5天内的分泌情况。两组中，培养的第2天至第3天，孵育培养基中IR-PRL的释放量达到每24小时间隔的最大值，足月分娩组为145.2±14.0 ng/ml/10 mg湿重（均值±标准误；n = 7），未足月分娩组为101.5±16.3 ng/ml/10 mg湿重（均值±标准误；n = 4）。两组在前3天IR-PRL的释放量无显著差异。然而，未足月分娩组释放的IR-PRL量是足月分娩组的50%至70%。两组中IR-PRL的组织含量在3.0至5.0 ng/ml/10 mg湿重之间。从这些结果得出以下结论：1）我们的人蜕膜组织外植体培养系统产生的IR-PRL比先前报道的要多得多；2）未足月分娩时IR-PRL的分泌量低于足月分娩；3）由于每24小时间隔内IR-PRL的组织含量非常少，应特别强调IR-PRL的产生和释放在人蜕膜组织中是同时发生的。
