Kubota T, Yaoi Y, Suzuki A, Nishi N, Kumasaka T, Saito M
Nihon Sanka Fujinka Gakkai Zasshi. 1981 Jan;33(1):51-9.
This study was conducted to examine the release of immunoreactive prolactin (IR-PRL) in vitro by 14 cases of decidual obtained from women with 6 to 13 weeks of gestation. 1) The dilution curve of IR-PRL released from the decidua was parallel to the pituitary standard PRL. 2) A highly significant rise of IR-PRL during the incubation of the decidua was found. In decidua of 11-13 weeks of gestation, the summarised IR-PRL level during 90 minutes was the highest, viz. 359.4 +/- 87.4 ng/0.1 g wet weight. 3) The villi showed scarcely any IR-PRL release, and the accumulated IR-PRL level was 2.4% of the decidual incubation value. Little IR-PRL release was found in the incubation of endometrium of luteal phase. 4) The temperature and pH of the medium influenced the IR-PRL release, but dopamine, bromocriptine and somatostatin had no effects on the PRL releasing activity of the decidua. 5) According to the gel filtration, the elution pattern of IR-PRL released from the decidua was similar to that of the amniotic fluid rather than that of the maternal plasma. From the above results, we suggested that the decidua was the original source of the PRL released in the amniotic fluid.
本研究旨在检测14例妊娠6至13周妇女的蜕膜组织在体外释放免疫反应性催乳素（IR-PRL）的情况。1）蜕膜释放的IR-PRL稀释曲线与垂体标准PRL平行。2）发现蜕膜孵育期间IR-PRL显著升高。在妊娠11至13周的蜕膜中，90分钟内的IR-PRL总量最高，即359.4±87.4 ng/0.1 g湿重。3）绒毛几乎不释放IR-PRL，累积的IR-PRL水平为蜕膜孵育值的2.4%。黄体期子宫内膜孵育时发现IR-PRL释放很少。4）培养基的温度和pH影响IR-PRL释放，但多巴胺、溴隐亭和生长抑素对蜕膜的PRL释放活性无影响。5）根据凝胶过滤结果，蜕膜释放的IR-PRL洗脱模式与羊水相似，而与母体血浆不同。根据上述结果，我们认为蜕膜是羊水中释放的PRL的原始来源。
