Queiroz Maiara I C, Sales Marcos V S, Barros Ellen Dos Santos Silva, D' Amato Flávio O S, Gonçalves Camilla M, Ursulino Jeferson S, Bueno Nassib B, Marinho Chiara, Rocha Ueslen, Aquino Thiago M, Fonseca Eduardo Jorge S, Borbely Alexandre U, Oliveira Helena C F, Santos Josué Carinhanha C, Leite Ana Catarina R
Instituto de Química e Biotecnologia, Universidade Federal de Alagoas (UFAL), Alagoas, Brazil.
Instituto de Física, Universidade Federal de Alagoas (UFAL), Alagoas, Brazil.
J Hazard Mater. 2025 Jul 15;492:138088. doi: 10.1016/j.jhazmat.2025.138088. Epub 2025 Mar 29.
Recent findings indicate elevated levels of total Hg in the waters of the Lagoa Mundaú-Manguaba Estuarine Complex (CELMM, Maceió-AL, Brazil) and the biological fluids of fishermen that live near CELMM. This study assessed Hg levels in whole blood and the functionality, structure, morphology, and oxidative stress of blood cells from fishermen (n = 60) compared with control volunteers (n = 65). A systematic assessment was performed, and our results revealed increased Hg concentration in fishermen's blood. Erythrocyte functionality showed a 39 % decrease in O uptake. For peripheral blood mononuclear cells (PBMCs), ROS generation demonstrated an 87 and 116 % increase in O and HO production, respectively, confirmed by fluorescence microscopy. Scanning electron microscopy showed increased roughness in the PBMCs membrane. Secondary oxidative stress markers revealed a reduction in the GSH/GSSG ratio and thiol content. MDA production increased by 89 %, while antioxidant enzyme activities showed a 159 and 22 % increase in SOD and CAT, respectively; otherwise, a depletion of 33 % in GPx. The metabolomic profile exhibited changes in essential amino acids for GSH formation, and energy pathways were modified. Finally, our data indicates that exposure to a polluted environment alters redox status, leading to compromised function and structure of blood cells.
最近的研究结果表明，蒙道乌-曼瓜巴泻湖河口复合体（CELMM，位于巴西阿拉戈斯州马塞约）水域以及居住在CELMM附近的渔民生物体液中的总汞含量升高。本研究评估了60名渔民与65名对照志愿者的全血汞含量以及血细胞的功能、结构、形态和氧化应激情况。进行了系统评估，结果显示渔民血液中的汞浓度升高。红细胞功能显示氧气摄取量减少了39%。对于外周血单核细胞（PBMC），通过荧光显微镜证实，活性氧生成分别显示超氧阴离子（O）和过氧化氢（HO）生成增加了87%和116%。扫描电子显微镜显示PBMC细胞膜粗糙度增加。二级氧化应激标志物显示谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值和硫醇含量降低。丙二醛（MDA）生成增加了89%，而抗氧化酶活性分别显示超氧化物歧化酶（SOD）和过氧化氢酶（CAT）增加了159%和22%；否则，谷胱甘肽过氧化物酶（GPx）减少了33%。代谢组学图谱显示参与谷胱甘肽形成的必需氨基酸发生变化，能量途径也被改变。最后，我们的数据表明，暴露于污染环境会改变氧化还原状态，导致血细胞的功能和结构受损。
