Villa Roberto Edoardo, Azimonti Giovanna, Bonos Eleftherios, Christensen Henrik, Durjava Mojca, Dusemund Birgit, Gehring Ronette, Glandorf Boet, Kouba Maryline, López-Alonso Marta, Marcon Francesca, Nebbia Carlo, Pechová Alena, Prieto-Maradona Miguel, Röhe Ilen, Theodoridou Katerina, Tosti Luca, Anguita Montserrat, Bozzi Cionci Nicole, Firmino Joana P, Innocenti Matteo L, Tarrés-Call Jordi, Pettenati Elisa
EFSA J. 2025 Apr 2;23(4):e9327. doi: 10.2903/j.efsa.2025.9327. eCollection 2025 Apr.
Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of the feed additive consisting of l-tryptophan produced by fermentation with a genetically modified strain of (KCCM 80346) when used as a nutritional additive in feed and water for drinking for all animal species and categories. Viable cells of the production strain and its DNA were not detected in the additive. l-Tryptophan manufactured by fermentation using KCCM 80346 does not give rise to any safety concern with regard to the genetic modification of the production strain. The use of l-tryptophan (≥ 98%) produced with KCCM 80346 to supplement feed is safe for non-ruminant species. There may be a risk for an increased production of toxic metabolites when unprotected tryptophan is used in ruminants. The FEEDAP Panel has concerns on the use of l-tryptophan in water for drinking. The use of l-tryptophan produced with KCCM 80346 in animal nutrition raises no safety concerns to consumers of products from animals receiving the additive and to the environment. l-tryptophan produced with KCCM 80346 is not irritant to the eyes and skin, and it is not a skin sensitiser. The additive l-tryptophan is regarded as an effective source of the amino acid l-tryptophan for all non-ruminant species. To be as efficacious in ruminants as in non-ruminants, it should be protected from ruminal degradation.
应欧盟委员会的要求，欧洲食品安全局（EFSA）被要求就一种由基因改造的（KCCM 80346）菌株发酵生产的L-色氨酸饲料添加剂在所有动物种类和类别用作饲料和饮用水中的营养添加剂时的安全性和有效性发表科学意见。在该添加剂中未检测到生产菌株的活细胞及其DNA。使用KCCM 80346发酵生产的L-色氨酸不会因生产菌株的基因改造而引起任何安全问题。使用KCCM 80346生产的L-色氨酸（≥98%）补充饲料对非反刍动物是安全的。在反刍动物中使用未受保护的色氨酸时，可能存在有毒代谢物产量增加的风险。饲料添加剂和动物营养产品委员会对在饮用水中使用L-色氨酸表示关切。在动物营养中使用KCCM 80346生产的L-色氨酸对接受该添加剂的动物产品消费者和环境不会引起安全问题。KCCM 80346生产的L-色氨酸对眼睛和皮肤无刺激性，且不是皮肤致敏剂。添加剂L-色氨酸被认为是所有非反刍动物氨基酸L-色氨酸的有效来源。要在反刍动物中与在非反刍动物中一样有效，应防止其在瘤胃中降解。
