Jensson H, Eriksson L C, Mannervik B
FEBS Lett. 1985 Jul 22;187(1):115-20. doi: 10.1016/0014-5793(85)81225-4.
Isoenzymes of glutathione transferase were shown to occur at selectively altered levels in rat hepatocyte nodules produced by 2-acetylaminofluorene treatment. Changes were measured by different substrates, antibodies raised against purified glutathione transferases, and by purification of the major isoenzymes. Isoenzymes composed of subunits 1, 2 and 3, expressed in normal liver tissue, all occurred at increased concentrations in nodules, whereas the level of transferase 4-4 was decreased. The most conspicuous change was the appearance of glutathione transferase 7-7 (or transferase P), the concentration of which in negligible in normal liver.
谷胱甘肽转移酶的同工酶在经2-乙酰氨基芴处理产生的大鼠肝细胞结节中呈现出选择性改变的水平。通过不同的底物、针对纯化的谷胱甘肽转移酶产生的抗体以及主要同工酶的纯化来测定变化。由亚基1、2和3组成的同工酶在正常肝组织中表达，在结节中的浓度均升高，而转移酶4-4的水平则降低。最显著的变化是谷胱甘肽转移酶7-7（或转移酶P）的出现，其在正常肝脏中的浓度可忽略不计。
