Cohen J, Fehilly C B, Walters D E
Fertil Steril. 1985 Aug;44(2):254-62. doi: 10.1016/s0015-0282(16)48747-9.
Spermatozoa from patients who underwent in vitro fertilization (IVF) therapy were prepared free from seminal plasma with the use of IVF culture medium supplemented with 8% human serum. Samples were then stored either at room temperature or in a refrigerator, and their motility and ability to penetrate zona-free hamster eggs were assessed every second day. With storage at room temperature, motility declined by less than half over the first 14 days, with some samples still active 20 days after preparation. The ability of the samples to penetrate hamster eggs was unchanged during the first 6 days of storage, and most of the samples still had positive tests after 14 days. Some spermatozoa could still penetrate after 17 days. Room-temperature-stored spermatozoa were still able to fertilize human oocytes 5 days after preparation. With storage in a refrigerator motility declined rapidly, and few sperm were motile after 14 days. However, these samples penetrated relatively more hamster eggs after 14 days' storage than room-temperature-stored samples did. Spermatozoa stored overnight in a refrigerator had significantly higher hamster egg penetration rates than spermatozoa stored overnight at room temperature. After storage overnight at room temperature, false-positive and false-negative results of hamster egg penetration tests were common in relation to IVF outcome; after refrigerated storage, no false-negative results were found.
使用添加了8%人血清的体外受精（IVF）培养基，对接受体外受精治疗的患者的精子进行去精浆处理。然后将样本分别储存在室温或冰箱中，每隔一天评估其活力以及穿透去透明带仓鼠卵的能力。在室温下储存时，在前14天内活力下降不到一半，一些样本在制备后20天仍有活性。样本穿透仓鼠卵的能力在储存的前6天没有变化，大多数样本在14天后仍有阳性检测结果。一些精子在17天后仍能穿透。室温储存的精子在制备后5天仍能够使人类卵母细胞受精。储存在冰箱中时，活力迅速下降，14天后几乎没有精子具有活力。然而，这些样本在储存14天后比室温储存的样本穿透相对更多的仓鼠卵。在冰箱中过夜储存的精子比在室温下过夜储存的精子具有显著更高的仓鼠卵穿透率。在室温下过夜储存后，仓鼠卵穿透试验的假阳性和假阴性结果与体外受精结果相关，较为常见；冷藏储存后，未发现假阴性结果。
