Li Tao, Shafiul Alam Mohammad, Yang Yu, Mohammad Al-Amin Hasan, Rahman Mezanur, Islam Farzana, Conte Matthew A, Price Dana C, Hang Jun
Viral Diseases Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States.
Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
PeerJ. 2025 Mar 31;13:e19180. doi: 10.7717/peerj.19180. eCollection 2025.
Bangladesh has a warm climate and landscapes favourable for the proliferation of mosquitoes. Mosquito-borne pathogens including malaria and arthropod-borne viruses (arboviruses) remain a serious threat to the public health requiring constant vector control and disease surveillance. From November 2018 to April 2019, mosquitoes were collected in three unions in the Ramu Upazila (sub-district) of Cox's Bazar District, Bangladesh. The mosquito specimens were combined into pools based on date of collection, household ID, and sex. Metagenome next-generation sequencing was conducted to elucidate diversity of virus sequences in each pool. Homology-based taxonomic classification and phylogenetic analyses identified a broad diversity of putative viruses from 12 known families, with additional unclassified viruses also likely present. Analysis of male mosquitoes showed some of these viruses are likely capable of being vertically transmitted. Moreover, many of the assembled virus sequences share homology and phylogenetic affinity with segments in sequenced genomes, and may represent endogenous viral elements derived from a past evolutionary relationship between these putative viruses and their mosquito hosts.
孟加拉国气候温暖，地貌有利于蚊子繁殖。包括疟疾和节肢动物传播病毒（虫媒病毒）在内的蚊媒病原体仍然对公众健康构成严重威胁，需要持续进行病媒控制和疾病监测。2018年11月至2019年4月，在孟加拉国科克斯巴扎尔区拉穆乡（分区）的三个联合乡收集了蚊子。根据收集日期、家庭ID和性别将蚊子标本合并成池。进行宏基因组下一代测序以阐明每个池中病毒序列的多样性。基于同源性的分类学分类和系统发育分析确定了来自12个已知科的多种假定病毒，可能还存在其他未分类的病毒。对雄蚊的分析表明，其中一些病毒可能能够垂直传播。此外，许多组装的病毒序列与已测序基因组中的片段具有同源性和系统发育亲和力，可能代表了这些假定病毒与其蚊子宿主过去进化关系中衍生的内源性病毒元件。
