Sivakoses Anutr, Marcarian Haley Q, Arias Anika M, Lam Alice R, Ihedioha Olivia C, Santamaria-Barria Juan A, Gurtner Geoffrey C, Bothwell Alfred L M
Arizona Cancer Center, University of Arizona, Tucson, Arizona, United States.
Department of Pathology, Microbiology, and Immunology, University of Nebraska Medical Center, Omaha, Nebraska, United States.
PeerJ. 2025 Mar 31;13:e19236. doi: 10.7717/peerj.19236. eCollection 2025.
Trogocytosis is the process by which a recipient cell siphons small membrane fragments and proteins from a donor cell and can be utilized by cancer cells to avoid immune detection. We observed lymphocyte specific protein expressed by triple negative breast cancer (TNBC) cells immunofluorescence imaging of patient samples. Image analysis of Cluster of Differentiation 45RA (CD45RA) expression, a naïve T cell specific protein, revealed that all stages of TNBCs express CD45RA. Flow cytometry revealed TNBC cells trogocytose CD45 protein from T cells. We also showed that the acquisition of these lymphoid markers is contact dependent. Confocal and super-resolution imaging further revealed CD45+ spherical structures containing T cell genomic DNA inside TNBC cells after co-culture. Trogocytosis between T cells and TNBC cells altered tumor cell expression of , the gene that encodes for CD45. Our results revealed that CD45 is obtained by TNBC cells from T cells trogocytosis and that TNBC cells express CD45 intracellularly and on the membrane.
胞啃作用是指受体细胞从供体细胞中汲取小的膜片段和蛋白质的过程，癌细胞可利用这一过程来逃避免疫检测。我们通过对患者样本进行免疫荧光成像，观察到三阴性乳腺癌（TNBC）细胞表达淋巴细胞特异性蛋白。对幼稚T细胞特异性蛋白分化簇45RA（CD45RA）表达的图像分析显示，TNBC的各个阶段均表达CD45RA。流式细胞术显示TNBC细胞从T细胞中胞啃CD45蛋白。我们还表明，这些淋巴标记物的获取是接触依赖性的。共培养后，共聚焦和超分辨率成像进一步显示TNBC细胞内含有T细胞基因组DNA的CD45 +球形结构。T细胞与TNBC细胞之间的胞啃作用改变了编码CD45的基因的肿瘤细胞表达。我们的结果表明，TNBC细胞通过胞啃作用从T细胞获得CD45，并且TNBC细胞在细胞内和细胞膜上均表达CD45。
