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肿瘤类器官与先进技术的结合：肿瘤演变和治疗反应的强大平台（综述）

Combination of tumor organoids with advanced technologies: A powerful platform for tumor evolution and treatment response (Review).

作者信息

Wu Ying, Zhang Fan, Du Furong, Huang Juan, Wei Shuqing

机构信息

Department of Obstetrics and Gynecology, The 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan 650032, P.R. China.

Department of Comprehensive Medicine, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi 030013, P.R. China.

出版信息

Mol Med Rep. 2025 Jun;31(6). doi: 10.3892/mmr.2025.13505. Epub 2025 Apr 4.

DOI:10.3892/mmr.2025.13505
PMID:40183402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11976518/
Abstract

Malignant tumors notably decrease life expectancy. Despite advances in cancer diagnosis and treatment, the mechanisms underlying tumorigenesis, progression and drug resistance have not been fully elucidated. An emerging method to study tumors is tumor organoids, which are a three‑dimensional miniature structure. These retain the patient‑specific tumor heterogeneity while demonstrating the histological, genetic and molecular features of original tumors. Compared with conventional cancer cell lines and animal models, patient‑derived tumor organoids are more advanced at physiological and clinical levels. Their synergistic combination with other technologies, such as organ‑on‑a‑chip, 3D‑bioprinting, tissue‑engineered cell scaffolds and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‑associated protein 9, may overcome limitations of the conventional 3D organoid culture and result in the development of more appropriate model systems that preserve the complex tumor stroma, inter‑organ and intra‑organ communications. The present review summarizes the evolution of tumor organoids and their combination with advanced technologies, as well as the application of tumor organoids in basic and clinical research.

摘要

恶性肿瘤显著缩短预期寿命。尽管癌症诊断和治疗取得了进展，但肿瘤发生、发展和耐药的机制尚未完全阐明。一种新兴的肿瘤研究方法是肿瘤类器官，它是一种三维微型结构。这些类器官保留了患者特异性的肿瘤异质性，同时展现出原发肿瘤的组织学、遗传学和分子特征。与传统癌细胞系和动物模型相比，患者来源的肿瘤类器官在生理和临床层面更为先进。它们与其他技术（如芯片器官、3D生物打印、组织工程细胞支架和规律成簇间隔短回文重复序列/CRISPR相关蛋白9）的协同结合，可能克服传统3D类器官培养的局限性，并促使开发出更合适的模型系统，以保留复杂的肿瘤基质、器官间和器官内通讯。本综述总结了肿瘤类器官的发展历程及其与先进技术的结合，以及肿瘤类器官在基础和临床研究中的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/f5b78e4fee5f/mmr-31-06-13505-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/761532a63bef/mmr-31-06-13505-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/0fc3731ad13d/mmr-31-06-13505-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/76ee2e58dcc2/mmr-31-06-13505-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/66144c705f17/mmr-31-06-13505-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/83b1fdb515de/mmr-31-06-13505-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/f5b78e4fee5f/mmr-31-06-13505-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/761532a63bef/mmr-31-06-13505-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/0fc3731ad13d/mmr-31-06-13505-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/76ee2e58dcc2/mmr-31-06-13505-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/66144c705f17/mmr-31-06-13505-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/83b1fdb515de/mmr-31-06-13505-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37ce/11976518/f5b78e4fee5f/mmr-31-06-13505-g05.jpg

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本文引用的文献

1
Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine.类器官：在疾病模型、药物发现、精准医学和再生医学中的发展与应用。
MedComm (2020). 2024 Sep 21;5(10):e735. doi: 10.1002/mco2.735. eCollection 2024 Oct.
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Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.2022 年全球癌症统计数据：全球 185 个国家和地区 36 种癌症的发病率和死亡率全球估计数。
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Converging bioprinting and organoids to better recapitulate the tumor microenvironment.
融合生物打印和类器官以更好地模拟肿瘤微环境。
Trends Biotechnol. 2024 May;42(5):648-663. doi: 10.1016/j.tibtech.2023.11.006. Epub 2023 Dec 9.
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Recent advances in lung organoid development and applications in disease modeling.肺类器官的最新进展及其在疾病建模中的应用。
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Pharmaco-proteogenomic characterization of liver cancer organoids for precision oncology.肝癌类器官的药物保护基因组特征分析用于精准肿瘤学。
Sci Transl Med. 2023 Jul 26;15(706):eadg3358. doi: 10.1126/scitranslmed.adg3358.
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Emerging organoid-immune co-culture models for cancer research: from oncoimmunology to personalized immunotherapies.新兴的类器官-免疫共培养模型在癌症研究中的应用：从肿瘤免疫学到个体化免疫治疗。
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Precision medicine applied to metastatic colorectal cancer using tumor-derived organoids and in-vitro sensitivity testing: a phase 2, single-center, open-label, and non-comparative study.精准医学在转移性结直肠癌中的应用：基于肿瘤衍生类器官和体外药敏检测的Ⅱ期、单中心、开放标签、非对照研究。
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