Wetzel M C, Charlemagne J
Dev Comp Immunol. 1985 Spring;9(2):261-70. doi: 10.1016/0145-305x(85)90117-x.
Natural anti-DNP antibodies were isolated by affinity chromatography from individual sera of three Cyprinid fish species (carp, goldfish and tench) and their electrofocusing (IEF) spectra were analysed in reducing conditions. In addition, immune anti-penicillin and anti-BSA antibodies were isolated from individual and pooled tench sera, and studied by IEF techniques on reduced samples. Diversity rates appeared to be rather low in the three fish species, and striking similarities arose between individuals of a same species. These results can be interpreted by the existence of particular selective pressures operating in poikilothermic species as it was already suggested by Du Pasquier. No enhancement of antibody heterogeneity could be detected in the tetraploid (carp and goldfish) species. This result is also in accordance with the selection of a restricted germ-line determined antibody repertoire in lower vertebrates.
通过亲和层析从三种鲤科鱼类（鲤鱼、金鱼和丁鱥）的个体血清中分离出天然抗二硝基苯酚（DNP）抗体，并在还原条件下分析其等电聚焦（IEF）光谱。此外，从丁鱥个体血清和混合血清中分离出免疫抗青霉素和抗牛血清白蛋白（BSA）抗体，并通过IEF技术对还原样品进行研究。这三种鱼类的抗体多样性率似乎相当低，同一物种的个体之间存在显著的相似性。正如杜·帕斯奎尔之前所指出的，这些结果可以通过变温动物中存在特定的选择压力来解释。在四倍体物种（鲤鱼和金鱼）中未检测到抗体异质性的增强。这一结果也与在低等脊椎动物中选择有限的种系决定抗体库相一致。
