Allan A, Jewell D P
Digestion. 1985;32(1):25-9. doi: 10.1159/000199212.
Rectal biopsies were maintained in organ culture over a 24-hour culture period, with good preservation of histological architecture. A filtrate containing Clostridium difficile toxin significantly inhibited the rise in epithelial alkaline phosphatase activity normally seen during culture. This effect was abolished by pre-incubation of the filtrate with Clostridium sordellii antitoxin, or heat inactivation. This effect is most probably due to a toxin of C. difficile. The method provides a new quantitative approach to the study of luminal toxins as possible pathogenic agents in idiopathic inflammatory diseases of the colon.
直肠活检组织在器官培养中维持24小时培养期，组织学结构保存良好。含有艰难梭菌毒素的滤液显著抑制了培养过程中正常出现的上皮碱性磷酸酶活性的升高。用索氏梭菌抗毒素预孵育滤液或热灭活可消除这种作用。这种作用很可能是由于艰难梭菌的一种毒素。该方法为研究管腔毒素作为结肠特发性炎症性疾病可能的病原体提供了一种新的定量方法。
