Allan A, Jewell D P
Gut. 1983 Sep;24(9):812-7. doi: 10.1136/gut.24.9.812.
An organ culture method for the maintenance of rectal biopsies over a period of 24 hours is described. Good preservation of histological architecture and continued crypt cell proliferation were shown over the culture period. The colonic enzyme alkaline phosphatase was found to rise over the period of culture. This rise was dependent upon continued protein synthesis by the cell. Changes in alkaline phosphatase activity during culture in biopsies from patients with ulcerative colitis and Crohn's disease are reported. This organ culture system and the measurement of alkaline phosphatase activity during culture provides a new approach to the assessment of luminal antigens as possible effectors of colonic epithelial cell damage.
本文描述了一种用于维持直肠活检组织24小时的器官培养方法。在培养期间，组织学结构得到良好保存，隐窝细胞持续增殖。发现结肠酶碱性磷酸酶在培养期间升高。这种升高依赖于细胞持续的蛋白质合成。报告了溃疡性结肠炎和克罗恩病患者活检组织在培养过程中碱性磷酸酶活性的变化。这种器官培养系统以及培养期间碱性磷酸酶活性的测定为评估腔内抗原作为结肠上皮细胞损伤的可能效应物提供了一种新方法。
