Reversibility of antibiotic incorporation in large organelles of kidney cell.

Large organelles, namely mitochondria and lysosomes, were fractionated from rat kidney. Antibiotics were added to the fraction and recombinated with no antibiotic-containing supernatant fraction. Tetracycline was quickly dissolved from the large organelle fraction. Cephaloridine had more affinity to organelles than tetracycline. Binding of amikacin was strongest and partly irreversible when added in vivo.