Zhang Yuan, Li Bingjie, Li Jie, Zhao Jing, Li Yan, Kang Shan
Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Department of Obstetrics and Gynecology, Hebei General Hospital, Shijiazhuang, China.
Biomark Med. 2025 May;19(9):329-339. doi: 10.1080/17520363.2025.2483495. Epub 2025 Apr 7.
Cervical cancer (CC) is a leading cause of cancer-related death in women. The N-myc down-stream regulatory gene (NDRG) family has an unclear prognostic role in CC.
We analyzed NDRG mRNA and protein levels in CC using public databases. And NDRG1 expression was verified through immunohistochemistry in clinical samples. Additionally, we utilized other bioinformatics tools to analyze the correlations between NDRG and survival, as well as immune infiltration.
NDRG1 was elevated, and NDRG2 was reduced in CC tissues. High NDRG1 and low NDRG2/3 correlated with poorer survival and were associated with reduced immune cell infiltration, particularly CD8+ T cells. Genetic alterations in NDRG1/2/3 were primarily amplifications, while DNA hypomethylation of NDRG1 in CC tissues, particularly at specific CpG sites, was associated with prognosis. PPI and enrichment analyses implicated NDRGs in metabolic processes, HIF-1 signaling, and immune regulation, underscoring their roles in CC progression and prognosis.
NDRG1/2 present potential as new prognostic biomarkers, shedding light on therapeutic targets for CC.
宫颈癌（CC）是女性癌症相关死亡的主要原因。N-myc下游调控基因（NDRG）家族在CC中的预后作用尚不清楚。
我们使用公共数据库分析了CC中NDRG的mRNA和蛋白质水平。并通过免疫组织化学在临床样本中验证了NDRG1的表达。此外，我们利用其他生物信息学工具分析了NDRG与生存以及免疫浸润之间的相关性。
CC组织中NDRG1升高，NDRG2降低。高NDRG1和低NDRG2/3与较差的生存相关，并与免疫细胞浸润减少有关，尤其是CD8+T细胞。NDRG1/2/3的基因改变主要是扩增，而CC组织中NDRG1的DNA低甲基化，特别是在特定的CpG位点，与预后相关。蛋白质-蛋白质相互作用（PPI）和富集分析表明NDRG参与代谢过程、缺氧诱导因子-1（HIF-1）信号传导和免疫调节，突出了它们在CC进展和预后中的作用。
NDRG1/2具有作为新的预后生物标志物的潜力，为CC的治疗靶点提供了线索。
