Liu Chang, Luo Jiangli, Yang Demei, Liu Xiongwei, Zhou Sixuan, Zhou Ying
School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation, Guiyang, China.
Front Microbiol. 2025 Mar 21;16:1570230. doi: 10.3389/fmicb.2025.1570230. eCollection 2025.
Endophytic and rhizosphere microorganisms play crucial roles in influencing the quality and secondary metabolite accumulation of traditional Chinese medicinal.
Endophytic and rhizosphere microorganisms play crucial roles in influencing the quality and secondary metabolite accumulation of traditional Chinese medicinal.
A total of 8,514,557 highquality reads were generated from 140 plant and soil sample in Sims based on high-throughput sequencing. The fungal species composition within the endophytic and rhizosphere soil samples of Sims is rich and varied, exhibiting notable disparities across different geographical regions of the plant. The alpha diversity and beta diversity indicated significant differences in microbial diversity and community structure between soil and plants. As for endophytic fungi, the dominant phyla in both plants and soil were Ascomycota and Basidiomycota, with different dominant genera between the two compartments. LEfSe analysis at the genus level identified 80 and 124 fungal indicator taxa associated with plants and soil, respectively, including , , , among others. Co-occurrence network analysis demonstrated intimate interactions among soil fungal microorganisms. Examination of soil physicochemical factors and the primary active constituent (bergenin) across different regions of Sims indicated that the highest bergenin concentration is found in the Guangxi region, whereas the Guizhou region boasts relatively abundant soil nutrient components. Correlation analysis revealed that , , , , and are correlated with soil physicochemistry or active compounds. These findings hint at a potential role for endophytic and rhizosphere microorganisms in the accumulation of active compounds within medicinal plants, thereby furnishing a scientific rationale for guiding the cultivation practices of Sims.
内生菌和根际微生物在影响中药质量和次生代谢产物积累方面发挥着关键作用。
内生菌和根际微生物在影响中药质量和次生代谢产物积累方面发挥着关键作用。
基于高通量测序，从喜树的140个植物和土壤样本中总共获得了8,514,557条高质量读数。喜树内生和根际土壤样本中的真菌物种组成丰富多样，在植物的不同地理区域表现出显著差异。α多样性和β多样性表明土壤和植物之间的微生物多样性和群落结构存在显著差异。至于内生真菌，植物和土壤中的优势门均为子囊菌门和担子菌门，两个区室中的优势属不同。属水平的LEfSe分析分别鉴定出80个和124个与植物和土壤相关的真菌指示类群，包括等等。共现网络分析表明土壤真菌微生物之间存在密切相互作用。对喜树不同区域的土壤理化因子和主要活性成分（岩白菜素）的检测表明，广西地区岩白菜素浓度最高，而贵州地区土壤养分成分相对丰富。相关性分析表明，、、、和与土壤理化性质或活性化合物相关。这些发现暗示内生菌和根际微生物在药用植物活性化合物积累中可能发挥作用，从而为指导喜树的栽培实践提供科学依据。
