Rayner J R, Cover W H, Martinez R J, Rittenberg S C
J Bacteriol. 1985 Aug;163(2):595-9. doi: 10.1128/jb.163.2.595-599.1985.
Outer membrane preparations of Bdellovibrio bacteriovorus grown intraperiplasmically on Escherichia coli containing OmpF were prepared by the Triton X-100 procedure of Schnaitman (J. Bacteriol. 108:545-552, 1971). They contained a protein that migrated to almost the same position as E. coli OmpF in sodium dodecyl sulfate-acrylamide gradient gel electrophoresis and to the same position as E. coli OmpF when urea was incorporated into the gel. The mobility of this protein increased relative to that of OmpC in urea-containing gels as does E. coli OmpF. However, the same protein was also produced during axenic growth and during intraperiplasmic growth on prey lacking OmpF. The peptide profile generated by partial proteolysis of this protein showed no homology to that produced from E. coli OmpF. We conclude that B. bacteriovorus synthesizes an OmpF-like protein. Previous claims that the bdellovibrio incorporates an intact E. coli OmpF are not consistent with these observations.
通过施奈特曼（《细菌学杂志》108:545 - 552，1971年）的Triton X - 100方法，制备了在含有OmpF的大肠杆菌周质内生长的食菌蛭弧菌的外膜制剂。它们含有一种蛋白质，在十二烷基硫酸钠 - 丙烯酰胺梯度凝胶电泳中，其迁移位置几乎与大肠杆菌OmpF相同，当凝胶中加入尿素时，其迁移位置与大肠杆菌OmpF相同。与大肠杆菌OmpF一样，在含尿素的凝胶中，这种蛋白质相对于OmpC的迁移率增加。然而，在无菌生长期间以及在缺乏OmpF的猎物上进行周质内生长期间，也会产生相同的蛋白质。该蛋白质经部分蛋白酶解产生的肽谱与大肠杆菌OmpF产生的肽谱没有同源性。我们得出结论，食菌蛭弧菌合成了一种类似OmpF的蛋白质。先前关于蛭弧菌整合完整大肠杆菌OmpF的说法与这些观察结果不一致。
