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嗜菌体蛭弧菌109D菌株对大肠杆菌外膜蛋白的获取

Acquisition of Escherichia coli outer membrane proteins by Bdellovibrio sp. strain 109D.

作者信息

Diedrich D L, Duran C P, Conti S F

出版信息

J Bacteriol. 1984 Jul;159(1):329-34. doi: 10.1128/jb.159.1.329-334.1984.

DOI:10.1128/jb.159.1.329-334.1984
PMID:6376474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215633/
Abstract

The ability of Bdellovibrio sp. to acquire the OmpF major outer membrane protein from its Escherichia coli prey was examined to determine if there were other outer membrane proteins which could or could not be acquired. Growth of bdellovibrios on mutant prey which were defective in the expression of outer membrane proteins revealed that Bdellovibrio sp. could acquire the OmpC protein in the absence of the OmpF protein. However, the OmpA, LamB, and protein 2 proteins could not be found in the Bdellovibrio Triton-insoluble outer membrane. The disappearance of the OmpF and OmpC proteins from the bdelloplast surface was measured, and it was determined that Bdellovibrio sp. exhibited a kinetic and temporal preference for the OmpF protein. Bdellovibrios could be grown on porin-deficient prey, and the progeny bdellovibrios possessed outer membranes with a protein mass deficiency.

摘要

研究了蛭弧菌属从其大肠杆菌猎物中获取OmpF主要外膜蛋白的能力,以确定是否存在其他能够或不能被获取的外膜蛋白。在表达外膜蛋白有缺陷的突变猎物上蛭弧菌的生长表明,在没有OmpF蛋白的情况下,蛭弧菌属可以获取OmpC蛋白。然而,在蛭弧菌的 Triton不溶性外膜中未发现OmpA、LamB和蛋白2。测量了bdelloplast表面OmpF和OmpC蛋白的消失情况,确定蛭弧菌属对OmpF蛋白表现出动力学和时间偏好。蛭弧菌可以在缺乏孔蛋白的猎物上生长,子代蛭弧菌的外膜存在蛋白质质量缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e82d/215633/dfcbd2037584/jbacter00230-0338-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e82d/215633/dfcbd2037584/jbacter00230-0338-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e82d/215633/dfcbd2037584/jbacter00230-0338-a.jpg

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