Nelson D R, Rittenberg S C
J Bacteriol. 1981 Sep;147(3):860-8. doi: 10.1128/jb.147.3.860-868.1981.
The composition of Bdellovibrio bacteriovorus lipopolysaccharide (LPS) was determined for cells grown axenically and intraperiplasmically on Escherichia coli or Pseudomonas putida. The LPS of axenically grown bdellovibrios contained glucose and fucosamine as the only detectable neutral sugar and amino sugar, and nonadecenoic acid (19:1) as the predominant fatty acid. Additional fatty acids, heptose, ketodeoxyoctoic acid, and phosphate were also detected. LPS from bdellovibrios grown intraperiplasmically contained components characteristic of both axenically grown bdellovibrios and the substrate cells. Substrate cell-derived LPS fatty acids made up the majority of the bdellovibrio LPS fatty acids and were present in about the same proportions as in the substrate cell LPS. Glucosamine derived from E. coli LPS amounted to about one-third of the hexosamine residues in intraperiplasmically grown bdellovibrio LPS. However, galactose, characteristic of the E. coli outer core and O antigen, was not detected in the bdellovibrio LPS, suggesting that only lipid A components of the substrate cell were incorporated. Substrate cell-derived and bdellovibrio-synthesized LPS materials were conserved in the B. bacteriovorus outer membrane for at least two cycles of intraperiplasmic growth. When bdellovibrios were grown on two different substrate cells successively, lipid A components were taken up from the second while the components incorporated from the lipid A of the first were conserved in the bdellovibrio LPS. The data show that substrate cell lipid A components were incorporated into B. bacteriovorus lipid A during intraperiplasmic growth with little or no change, and that these components, fatty acids and hexosamines, comprised a substantial portion of bdellovibrio lipid A.
测定了在无菌条件下以及在大肠杆菌或恶臭假单胞菌的周质空间内生长的食菌蛭弧菌脂多糖（LPS）的组成。无菌培养的蛭弧菌的LPS含有葡萄糖和岩藻糖胺，这是唯一可检测到的中性糖和氨基糖，并且以十九碳烯酸（19:1）作为主要脂肪酸。还检测到了其他脂肪酸、庚糖、酮脱氧辛酸和磷酸盐。在周质空间内生长的蛭弧菌的LPS含有无菌培养的蛭弧菌和底物细胞的特征性成分。底物细胞来源的LPS脂肪酸构成了蛭弧菌LPS脂肪酸的大部分，并且其比例与底物细胞LPS中的比例大致相同。来自大肠杆菌LPS的葡萄糖胺约占周质空间内生长的蛭弧菌LPS中己糖胺残基的三分之一。然而，在蛭弧菌LPS中未检测到大肠杆菌外核心和O抗原特有的半乳糖，这表明仅掺入了底物细胞的脂质A成分。底物细胞来源的和蛭弧菌合成的LPS物质在食菌蛭弧菌外膜中至少在两个周质生长周期中得以保留。当蛭弧菌先后在两种不同的底物细胞上生长时，脂质A成分从第二种底物细胞中摄取，而从第一种底物细胞脂质A中掺入的成分则保留在蛭弧菌LPS中。数据表明，在周质生长过程中，底物细胞脂质A成分几乎没有变化地掺入到食菌蛭弧菌脂质A中，并且这些成分，即脂肪酸和己糖胺，构成了蛭弧菌脂质A的很大一部分。
