Bakirhan Nurgul K, Sener Sıla Ozlem, Baran Merve Yuzbasioglu, Bahend Khadija, Ozgen Ufuk
University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey.
University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.
J AOAC Int. 2025 Sep 1;108(5):760-768. doi: 10.1093/jaoacint/qsaf032.
Miquelianin (MIQ) is a natural phenolic compound found in various plants, including Salvia species, with potential health benefits due to its antioxidant and anti-inflammatory properties.
This study presents an investigation of the electrochemical oxidation pathway and sensitive analysis of MIQ, which was isolated from the aerial parts of Calystegia silvatica (CS) that grows in Trabzon, Turkey.
Electrochemical determination methods have gained significant attention for the quantitative analysis of bioactive compounds due to their simplicity, sensitivity, and cost-effectiveness. The electrochemical determination of MIQ primarily involves the utilization of electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV).
Under optimum experimental conditions, a calibration plot for MIQ with LODs of 99.3 × 10-9 M and 145 × 10-9 M were obtained using DPV with glassy carbon electrodes (GCEs) and boron-doped diamond electrodes (BDDEs) in the range of 2.0 × 10-6 to 3.6 × 10-5 M. The presented method was validated and successfully performed for the determination of MIQ from plant extracts with excellent recovery values as 104.7, 101.8, and 102.1%.
This study explores the electrochemical oxidation pathway and sensitive analysis of MIQ, isolated from C. silvatica in Trabzon, Turkey. GCEs and BDDEs as carbon-based electrodes were used for the sensitive analysis of MIQ in phosphate buffer (PB) solution at pH 7.0 solution. MIQ was detected using GCEs and BDDEs with limits of 99.3 × 10-9 M and 145 × 10-9 M, respectively, within a concentration range of 2.0 × 10-6 to 3.6 × 10-5 M. The validated method demonstrated excellent recovery values of 104.7, 101.8, and 102.1% for determining MIQ from plant extracts.
This electrochemical method offers promising opportunities for accurate and sensitive analysis of MIQ. Continued research and technological advancements in this field can contribute to a deeper understanding of the electrochemical behavior of MIQ and facilitate its practical applications in pharmaceutical, food, and nutraceutical industries, promoting its utilization as a valuable bioactive compound.
杨梅素（MIQ）是一种天然酚类化合物，存在于包括丹参属植物在内的多种植物中，因其抗氧化和抗炎特性而具有潜在的健康益处。
本研究对从生长于土耳其特拉布宗的圆叶打碗花（CS）地上部分分离得到的MIQ进行了电化学氧化途径研究及灵敏分析。
电化学测定方法因其简便、灵敏且经济高效，在生物活性化合物的定量分析中受到了广泛关注。MIQ的电化学测定主要涉及循环伏安法（CV）和差分脉冲伏安法（DPV）等电化学技术的应用。
在最佳实验条件下，使用玻碳电极（GCEs）和硼掺杂金刚石电极（BDDEs），通过差分脉冲伏安法在2.0×10⁻⁶至3.6×10⁻⁵ M范围内获得了MIQ的校准曲线，检测限分别为99.3×10⁻⁹ M和145×10⁻⁹ M。所提出的方法经过验证，成功用于测定植物提取物中的MIQ，回收率极佳，分别为104.7%、101.8%和102.1%。
本研究探索了从土耳其特拉布宗的圆叶打碗花中分离得到的MIQ的电化学氧化途径及灵敏分析。作为碳基电极的GCEs和BDDEs用于在pH 7.0的磷酸盐缓冲液（PB）溶液中对MIQ进行灵敏分析。使用GCEs和BDDEs检测MIQ，在2.0×10⁻⁶至3.6×10⁻⁵ M的浓度范围内，检测限分别为99.3×10⁻⁹ M和145×10⁻⁹ M。经过验证的方法在测定植物提取物中的MIQ时显示出104.7%、101.8%和102.1%的极佳回收率。
这种电化学方法为MIQ的准确和灵敏分析提供了有前景的机会。该领域持续的研究和技术进步有助于更深入地理解MIQ的电化学行为，并促进其在制药、食品和营养保健品行业的实际应用，推动其作为一种有价值的生物活性化合物的利用。
