• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

可在一小部分脊髓背角神经元中驱动四环素调控基因表达的新型小鼠品系

New Mouse Lines That Drive Tetracycline-Controlled Gene Expression in a Small Subset of Spinal Cord Dorsal Horn Neurons.

作者信息

Fyrberg Eric, Learnard Heather, Lee Soojin, Jun Yong-Woo, Gao Fen-Biao

机构信息

RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605.

Department of Neurology, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605.

出版信息

eNeuro. 2025 Apr 22;12(4). doi: 10.1523/ENEURO.0441-24.2025. Print 2025 Apr.

DOI:10.1523/ENEURO.0441-24.2025
PMID:40199586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12014207/
Abstract

Mouse lines with tetracycline-controlled gene expression in specific neuronal populations provide valuable tools for studying their development, function, connectivity, and pathology in vivo. Our initial goal was to generate a mouse model that could express amyotrophic lateral sclerosis-associated genes specifically in spinal cord motor neurons under the control of the promoter. However, mice unexpectedly direct target gene expression in a small subset of dorsal horn neurons. These mice represent a new tool for scientists who are interested in studying these spinal cord neurons.

摘要

在特定神经元群体中具有四环素控制基因表达的小鼠品系为在体内研究它们的发育、功能、连接性和病理学提供了有价值的工具。我们最初的目标是生成一种小鼠模型，该模型可以在启动子的控制下在脊髓运动神经元中特异性地表达肌萎缩侧索硬化相关基因。然而，这些小鼠意外地在一小部分背角神经元中直接靶向基因表达。这些小鼠为对研究这些脊髓神经元感兴趣的科学家提供了一种新工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/a49c4ce7d023/eneuro-12-ENEURO.0441-24.2025-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/a298c5aa3098/eneuro-12-ENEURO.0441-24.2025-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/cdca423cc9ef/eneuro-12-ENEURO.0441-24.2025-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/6d91035d6541/eneuro-12-ENEURO.0441-24.2025-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/2d1e9ef5fab5/eneuro-12-ENEURO.0441-24.2025-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/a49c4ce7d023/eneuro-12-ENEURO.0441-24.2025-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/a298c5aa3098/eneuro-12-ENEURO.0441-24.2025-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/cdca423cc9ef/eneuro-12-ENEURO.0441-24.2025-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/6d91035d6541/eneuro-12-ENEURO.0441-24.2025-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/2d1e9ef5fab5/eneuro-12-ENEURO.0441-24.2025-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12014207/a49c4ce7d023/eneuro-12-ENEURO.0441-24.2025-g005.jpg

相似文献

1
New Mouse Lines That Drive Tetracycline-Controlled Gene Expression in a Small Subset of Spinal Cord Dorsal Horn Neurons.可在一小部分脊髓背角神经元中驱动四环素调控基因表达的新型小鼠品系
eNeuro. 2025 Apr 22;12(4). doi: 10.1523/ENEURO.0441-24.2025. Print 2025 Apr.
2
Kif1Bbeta isoform is enriched in motor neurons but does not change in a mouse model of amyotrophic lateral sclerosis.Kif1Bbeta 亚型在运动神经元中富集，但在肌萎缩侧索硬化症小鼠模型中无变化。
J Neurosci Res. 2003 Mar 1;71(5):732-9. doi: 10.1002/jnr.10517.
3
Glycine receptor channels in spinal motoneurons are abnormal in a transgenic mouse model of amyotrophic lateral sclerosis.脊髓运动神经元中的甘氨酸受体通道在肌萎缩侧索硬化症的转基因小鼠模型中异常。
J Neurosci. 2011 Feb 23;31(8):2815-27. doi: 10.1523/JNEUROSCI.2475-10.2011.
4
The Neurokinin-1 Receptor is Expressed with Gastrin-Releasing Peptide Receptor in Spinal Interneurons and Modulates Itch.神经激肽-1 受体与胃泌素释放肽受体在脊髓中间神经元中表达，并调节瘙痒。
J Neurosci. 2020 Nov 11;40(46):8816-8830. doi: 10.1523/JNEUROSCI.1832-20.2020. Epub 2020 Oct 13.
5
Contribution of dorsal horn CGRP-expressing interneurons to mechanical sensitivity.背角 CGRP 表达中间神经元对机械敏感性的贡献。
Elife. 2021 Jun 1;10:e59751. doi: 10.7554/eLife.59751.
6
Mammalian BarH1 confers commissural neuron identity on dorsal cells in the spinal cord.哺乳动物的BarH1赋予脊髓背侧细胞连合神经元特性。
J Neurosci. 2003 Mar 15;23(6):1987-91. doi: 10.1523/JNEUROSCI.23-06-01987.2003.
7
An astrocyte regenerative response from vimentin-containing cells in the spinal cord of amyotrophic lateral sclerosis's disease-like transgenic (G93A SOD1) mice.来自肌萎缩侧索硬化症样转基因（G93A SOD1）小鼠脊髓中含波形蛋白细胞的星形胶质细胞再生反应。
Neurodegener Dis. 2015;15(1):1-12. doi: 10.1159/000369466. Epub 2015 Jan 15.
8
Comparing effects of microgravity and amyotrophic lateral sclerosis in the mouse ventral lumbar spinal cord.比较微重力和肌萎缩侧索硬化症对小鼠腹侧腰脊髓的影响。
Mol Cell Neurosci. 2022 Jul;121:103745. doi: 10.1016/j.mcn.2022.103745. Epub 2022 Jun 2.
9
Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord.转录因子 Hb9 在发育中的小鼠脊髓神经胶质细胞谱系中表达。
eNeuro. 2022 Nov 3;9(6). doi: 10.1523/ENEURO.0214-22.2022. Print 2022 Nov-Dec.
10
Parvalbumin and calbindin D-28k immunoreactivity in transgenic mice with a G93A mutant SOD1 gene.携带G93A突变型SOD1基因的转基因小鼠中的小清蛋白和钙结合蛋白D-28k免疫反应性
Brain Res. 2006 Apr 14;1083(1):196-203. doi: 10.1016/j.brainres.2006.01.129. Epub 2006 Mar 20.

本文引用的文献

1
Spinal GABAergic neurons are under feed-forward inhibitory control driven by A and C fibers in Gad2 td-Tomato mice.Gad2 td-Tomato 小鼠的脊髓 GABA 能神经元受到 A 纤维和 C 纤维的前馈抑制控制。
Mol Pain. 2021 Jan-Dec;17:1744806921992620. doi: 10.1177/1744806921992620.
2
C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo.C9ORF72-ALS/FTD 相关多聚（GR）结合 Atp5a1 并在体内损害线粒体功能。
Nat Neurosci. 2019 Jun;22(6):851-862. doi: 10.1038/s41593-019-0397-0. Epub 2019 May 13.
3
Isolation and Characterization of Mouse Fibroblasts.
小鼠成纤维细胞的分离与鉴定
Methods Mol Biol. 2018;1809:59-67. doi: 10.1007/978-1-4939-8570-8_5.
4
Mouse models of ALS: Past, present and future.肌萎缩侧索硬化症的小鼠模型：过去、现在与未来
Brain Res. 2018 Aug 15;1693(Pt A):1-10. doi: 10.1016/j.brainres.2018.03.024. Epub 2018 Mar 22.
5
Decoding ALS: from genes to mechanism.解码肌萎缩侧索硬化症：从基因到机制
Nature. 2016 Nov 10;539(7628):197-206. doi: 10.1038/nature20413.
6
Neural circuits for pain: Recent advances and current views.疼痛的神经回路：最新进展与当前观点
Science. 2016 Nov 4;354(6312):578-584. doi: 10.1126/science.aaf8933.
7
TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.瞬时受体电位（TRP）通道相关因子是一个新型蛋白质家族，可调节瞬时受体电位M8型（TRPM8）通道的运输与活性。
J Cell Biol. 2015 Jan 5;208(1):89-107. doi: 10.1083/jcb.201402076.
8
Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia.微小RNA-124和AMPA受体的改变导致额颞叶痴呆的社会行为缺陷。
Nat Med. 2014 Dec;20(12):1444-51. doi: 10.1038/nm.3717. Epub 2014 Nov 17.
9
Neuronal Elav-like (Hu) proteins regulate RNA splicing and abundance to control glutamate levels and neuronal excitability.神经元 Elav 样（Hu）蛋白通过调节 RNA 剪接和丰度来控制谷氨酸水平和神经元兴奋性。
Neuron. 2012 Sep 20;75(6):1067-80. doi: 10.1016/j.neuron.2012.07.009.
10
Transcription factors: from enhancer binding to developmental control.转录因子：从增强子结合到发育控制。
Nat Rev Genet. 2012 Sep;13(9):613-26. doi: 10.1038/nrg3207. Epub 2012 Aug 7.