Press M F, Nousek-Goebl N A, Bur M, Greene G L
Am J Pathol. 1986 May;123(2):280-92.
The authors have used monoclonal estrophilin antibodies and the peroxidase antiperoxidase technique to characterize the distribution of estrogen receptor in the human vagina, uterus, and fallopian tubes. Exclusively nuclear localization of estrogen receptor was observed in epithelial cells, stromal cells of the lamina propria, and smooth muscle cells with both immunohistologic studies and immunoelectron-microscopy studies. The endometrium in various regions of the uterus (uterine isthmus, corpus, and fundus) was stained for estrogen receptor, with similar staining intensities in each of the respective cell types. There was no systematic regional variation in the staining intensity or distribution of cells with stained nuclei. The functionalis of the endometrium showed distinctive variation in the intensity of the staining for estrogen receptor with the menstrual cycle. The staining intensity of both endometrial epithelium and stroma was greatly reduced in the functionalis during the secretory phase. The vaginal epithelium did show some variation with the menstrual cycle, but it was much less than that observed in the functionalis of the endometrium. The basal cells of the vaginal stratified squamous epithelium, strongly stained during the proliferative phase, were less strongly stained during the secretory phase. No variation in the staining intensity for estrogen receptor was observed in different regions of the fallopian tube (isthmus, ampulla, and infundibulum), and the staining intensity varied only minimally with the menstrual cycle. The serosa of the female reproductive tract, connective tissues in the muscularis and in the vicinity of blood vessels, as well as neutrophils, eosinophils, mast cells, and lymphoid cells in the female genital tract were not stained for estrogen receptor.
作者使用单克隆雌激素受体抗体和过氧化物酶抗过氧化物酶技术来表征雌激素受体在人阴道、子宫和输卵管中的分布。免疫组织学研究和免疫电子显微镜研究均显示，雌激素受体仅定位于上皮细胞、固有层基质细胞和平滑肌细胞的细胞核中。子宫各区域（子宫峡部、宫体和宫底）的子宫内膜均进行了雌激素受体染色，各相应细胞类型的染色强度相似。细胞核染色细胞的染色强度或分布没有系统性的区域差异。子宫内膜功能层在月经周期中雌激素受体染色强度呈现出明显变化。在分泌期，子宫内膜功能层的上皮和基质的染色强度均大幅降低。阴道上皮在月经周期中也有一些变化，但远小于子宫内膜功能层所观察到的变化。阴道复层鳞状上皮的基底细胞在增殖期染色强烈，而在分泌期染色较弱。输卵管不同区域（峡部、壶腹部和漏斗部）未观察到雌激素受体染色强度的差异，且染色强度仅随月经周期有微小变化。女性生殖道的浆膜、肌层和血管附近的结缔组织，以及女性生殖道中的中性粒细胞、嗜酸性粒细胞、肥大细胞和淋巴细胞均未检测到雌激素受体染色。
