Bovine subclinical mastitis-associated methicillin-resistant , selective genotyping and antimicrobial susceptibility profile of the isolates in Kurdistan province of Iran.

BACKGROUND AND OBJECTIVES

is frequently involved in bovine subclinical mastitis worldwide. Besides, the methicillin-resistant (MRSA) carrier state of animals is a matter of worrisome. This study aimed to evaluate the frequency of MRSA, discriminatory geno-analysis and antibiotic resistance scheme of the strains isolated from bovine subclinical mastitis in Kurdistan province of Iran.

MATERIALS AND METHODS

A total of 283 samples were collected and analyzed for phenotypically and molecularly. SCC and types, and gene were evaluated using polymerase chain reaction (PCR). Finally, the restriction fragment length polymorphism (RFLP) patterns of types and the antimicrobial susceptibility profile of the isolates were assessed.

RESULTS

Among the 95 isolates of , 11 (11.57%) strains were recognized as MRSA. Six, one, and four SCC types represented for IVa, IVc, and V were determined, respectively, among which an individual IVa and V determinant harboured gene. Restriction digestion products of 490 bp, 680 bp, and 730 bp of bands were generated. Tobramycin, mupirocin, fusidic acid, clindamycin, and chloramphenicol were the most effective drugs against the MRSA isolates.

CONCLUSION

The detrimental involvement of in bovine subclinical mastitis is proved herein. Besides, the contribution of MRSA and potential contamination of milk and dairy products with the bacterium may impose a serious public health risk. This demands serious and long-lasting efforts to control the infection. The results may be effective in the implementation of accurate controlling strategies.