Fadhil Samar Abbas, Ali Mansour Jadaan
Department of Microbiology, College of Veterinary Medicine, University of Al-Qadisyiah, AL-Diwanyia, Iraq.
Open Vet J. 2025 Feb;15(2):660-667. doi: 10.5455/OVJ.2025.v15.i2.14. Epub 2025 Feb 28.
Canine leishmaniasis is an infectious protozoan disease transmitted by sand fly bites. The parasite infects the macrophages of vertebrates during the amastigote stage.
This study aimed to detect the causative species for infections between dogs with in Iraq and to determine the relationship between isolates that infect Iraqi animals and other animals worldwide.
Fifty dogs of various breeds, ranging from 1 month to 7 years old of both sexes. Then, blood samples were collected from the cephalic vein into an EDTA tube; DNA was extracted using a commercial kit. Afterward, conventional PCR was performed using universal primers to detect 18S in .
Most dog infections were caused by . The age of the infected dogs ranged from a few months to 7 years. Our results indicate that most infections with spp. occurred between older males and younger females. Most dogs were asymptomatic, and a small percentage exhibited clinical signs, such as skin lesions, rough coats, hair loss, and onychogryphosis. To confirm the results and gain insight into the origin of the positive isolates, 10 samples were sequenced. Interestingly, isolates were typical of those recorded in Brazil, France, and the USA, except for one isolate, which was slightly different from the detected isolates. Therefore, our results showed that most infections were between older males rather than younger females, and the DNA sequencing results indicated that only 1 isolate out of 10 had a different demographic origin.
Dogs in this study were infected mainly with without any clinical signs; most isolates resemble those recorded in Brazil, France, and the USA.
犬利什曼病是一种由白蛉叮咬传播的传染性原生动物疾病。该寄生虫在无鞭毛体阶段感染脊椎动物的巨噬细胞。
本研究旨在检测伊拉克犬类感染的致病物种，并确定感染伊拉克动物的分离株与全球其他动物分离株之间的关系。
选取50只不同品种、年龄在1个月至7岁之间的雌雄犬。然后，从头部静脉采集血液样本至乙二胺四乙酸（EDTA）管中；使用商业试剂盒提取DNA。之后，使用通用引物进行常规聚合酶链反应（PCR）以检测……中的18S。
大多数犬类感染是由……引起的。感染犬的年龄从几个月到7岁不等。我们的结果表明，大多数……属物种感染发生在年龄较大的雄性和较年轻的雌性之间。大多数犬没有症状，一小部分表现出临床症状，如皮肤病变、被毛粗糙、脱毛和爪甲异常。为了证实结果并深入了解阳性……分离株的来源，对10个样本进行了测序。有趣的是，……分离株与在巴西、法国和美国记录的典型分离株相同，只有一个分离株与检测到的分离株略有不同。因此，我们的结果表明，大多数感染发生在年龄较大的雄性而非较年轻的雌性之间，DNA测序结果表明10个分离株中只有1个具有不同的人口统计学来源。
本研究中的犬主要感染……且无任何临床症状；大多数分离株与在巴西、法国和美国记录的分离株相似。
