使用磁激活细胞分选法分离小鼠成纤维/脂肪生成祖细胞的方案。

Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting.

作者信息

Gao Jinghui, Zheng Yaochao, Sikal Aria, Sterling Elijah, Hankin Rachel, Yao Yao

机构信息

Regenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USA.

出版信息

STAR Protoc. 2025 Apr 8;6(2):103741. doi: 10.1016/j.xpro.2025.103741.

DOI:10.1016/j.xpro.2025.103741

PMID:40202840

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12008570/

Abstract

Fibro/adipogenic progenitors (FAPs) are mesenchymal progenitors that support muscle stem cell activation and modulate the extracellular matrix to facilitate muscle regeneration. Here, we present a protocol for the isolation of FAPs from mouse skeletal muscle. We describe steps for performing enzymatic digestion, mechanical dissociation, and magnetic-activated cell sorting (MACS). This protocol enables the isolation of FAPs with high yield and purity, facilitating research on muscle disorders and advancing muscle regeneration strategies.

摘要

成纤维/脂肪生成祖细胞（FAPs）是间充质祖细胞，可支持肌肉干细胞激活并调节细胞外基质以促进肌肉再生。在此，我们展示了一种从小鼠骨骼肌中分离FAPs的方案。我们描述了进行酶消化、机械解离和磁珠分选（MACS）的步骤。该方案能够以高产率和高纯度分离FAPs，有助于肌肉疾病的研究并推动肌肉再生策略的发展。