Neurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California San Diego, La Jolla, California 92093.
Neurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California San Diego, La Jolla, California 92093
J Neurosci. 2021 May 12;41(19):4160-4168. doi: 10.1523/JNEUROSCI.3003-20.2021. Epub 2021 Apr 23.
The brain functions through coordinated activity among distributed regions. Wide-field calcium imaging, combined with improved genetically encoded calcium indicators, allows sufficient signal-to-noise ratio and spatiotemporal resolution to afford a unique opportunity to capture cortex-wide dynamics on a moment-by-moment basis in behaving animals. Recent applications of this approach have been uncovering cortical dynamics at unprecedented scales during various cognitive processes, ranging from relatively simple sensorimotor integration to more complex decision-making tasks. In this review, we will highlight recent scientific advances enabled by wide-field calcium imaging in behaving mice. We then summarize several technical considerations and future opportunities for wide-field imaging to uncover large-scale circuit dynamics.
大脑通过分布区域之间的协调活动来发挥功能。宽场钙成像技术，结合改良的遗传编码钙指示剂，可提供足够的信噪比和时空分辨率，从而为在行为动物中逐时刻捕获皮质全范围动力学提供了独特的机会。这种方法的最近应用揭示了各种认知过程中前所未有的皮质动力学，从相对简单的感觉运动整合到更复杂的决策任务。在这篇综述中，我们将重点介绍宽场钙成像在行为小鼠中实现的最新科学进展。然后，我们总结了宽场成像揭示大规模电路动力学的几个技术考虑因素和未来机会。
