Solhaug Anita, Dowd Georgina C, Dayeh Vivian R, Sindre Hilde, Lee Lucy E J, Bols Niels C
Norwegian Veterinary Institute, 1433, Ås, Norway.
The New Zealand Institute for Plant and Food Research Limited, 293 Akersten Street, Nelson, 7010, New Zealand.
In Vitro Cell Dev Biol Anim. 2025 Apr 9. doi: 10.1007/s11626-025-01042-1.
There is a drive towards reducing animal experiments and developing robust biologically relevant in vitro models based on cell lines, including those derived from fish. At the time of writing, Cellosaurus, the knowledge base of current cell lines used in research, listed more than 900 fish cell lines in its database. One of the key challenges facing fish cell biology is the lack of fundamental technical information regarding the isolation, culture, and application of cell lines. Researchers often work in silos, encountering similar technical challenges, each spending significant time and resources overcoming the same issues for which solutions may not be readily accessible. Here, we share some of the key considerations for the isolation, culture, maintenance, and application of fish cell lines in toxicology, which we have encountered over our collective decades of experience.
目前有一种趋势，即减少动物实验，并基于细胞系开发强大的具有生物学相关性的体外模型，包括源自鱼类的细胞系。在撰写本文时，研究中使用的当前细胞系知识库Cellosaurus在其数据库中列出了900多种鱼类细胞系。鱼类细胞生物学面临的关键挑战之一是缺乏有关细胞系分离、培养和应用的基本技术信息。研究人员往往各自为政，面临相似的技术挑战，每个人都花费大量时间和资源来克服同样的问题，而这些问题的解决方案可能并不容易获得。在此，我们分享一些在毒理学中分离、培养、维持和应用鱼类细胞系的关键注意事项，这些是我们在几十年的共同经验中遇到的。
