Bols Niels C, Pham Phuc H, Dayeh Vivian R, Lee Lucy E J
Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada.
Faculty of Science, University of the Fraser Valley, V2S 7M8, Abbotsford, BC, Canada.
In Vitro Cell Dev Biol Anim. 2017 May;53(5):383-405. doi: 10.1007/s11626-017-0142-5. Epub 2017 Apr 3.
The literature on cell lines that have been developed from rainbow trout (RT) (Oncorhynchus mykiss) is reviewed to illustrate three new terms: invitromatics, invitrome, and invitroomics. Invitromatics is defined as the history, development, characterization, engineering, storage, and sharing of cell lines. RT invitromatics differs from invitromatics for humans and other mammals in several ways. Nearly all the RT cell lines have developed through spontaneous immortalization. No RT cell line undergoes senescence and can be described as being finite, whereas many human cell lines undergo senescence and are finite. RT cell lines are routinely grown at 18-22°C in free gas exchange with air in basal media developed for mammalian cells together with a supplement of fetal bovine serum. An invitrome is defined as the grouping of cell lines around a theme or category. The broad theme in this article is all the cell lines that have ever been created from O. mykiss, or in other words, the RT invitrome. The RT invitrome consists of approximately 55 cell lines. These cell lines can also be categorized on the basis of their storage and availability. A curated invitrome constitutes all the cell lines in a repository and for RT consists of 11 cell lines. These consist of epithelial cell lines, such as RTgill-W1, and fibroblast cell lines, such as RTG-2. RTG-2 can be purchased from a scientific company and constitutes the commercial RT invitrome. Cell lines that are exchanged between researchers are termed the informally shared invitrome and for RT consists of over 35 cell lines. Among these is the monocyte/macrophage cell line, RTS11. Cell lines whose existence is in doubt are termed the zombie invitrome, and for RT, approximately 12 cell lines are zombies. Invitroomics is the application of cell lines to a scientific problem or discipline. This is illustrated with the use of the RT invitrome in virology. Of the RT invitrome, RTG-2 was the most commonly used cell line to isolate viruses. Fifteen families of viruses were studied with RT invitrome. RT cell lines were best able to support replication of viruses from the Herpesviridae, Iridoviridae, Birnaviridae, Togaviridae, and Rhabdoviridae families.
本文回顾了从虹鳟（RT）（Oncorhynchus mykiss）培育出的细胞系文献，以阐述三个新术语：体外生物学、体外基因组和体外组学。体外生物学被定义为细胞系的历史、发展、特征描述、工程改造、储存和共享。虹鳟体外生物学在几个方面与人类和其他哺乳动物的体外生物学不同。几乎所有的虹鳟细胞系都是通过自发永生化发展而来的。没有虹鳟细胞系会经历衰老，可被描述为有限细胞系，而许多人类细胞系会经历衰老且是有限的。虹鳟细胞系通常在18 - 22°C下，在为哺乳动物细胞开发的基础培养基中与空气进行自由气体交换，并添加胎牛血清的条件下培养。体外基因组被定义为围绕一个主题或类别对细胞系进行的分组。本文的广泛主题是所有从虹鳟创建的细胞系，换句话说，就是虹鳟体外基因组。虹鳟体外基因组由大约55个细胞系组成。这些细胞系也可以根据其储存情况和可获取性进行分类。一个经过整理的体外基因组构成了一个储存库中的所有细胞系，对于虹鳟来说，由11个细胞系组成。这些包括上皮细胞系，如RTgill - W1，和成纤维细胞系，如RTG - 2。RTG - 2可以从一家科学公司购买，构成商业虹鳟体外基因组。研究人员之间交换的细胞系被称为非正式共享体外基因组，对于虹鳟来说，由超过35个细胞系组成。其中包括单核细胞/巨噬细胞系RTS11。存在疑问的细胞系被称为僵尸体外基因组，对于虹鳟来说，大约有12个细胞系是僵尸细胞系。体外组学是将细胞系应用于科学问题或学科。这通过在病毒学中使用虹鳟体外基因组得到了说明。在虹鳟体外基因组中，RTG - 2是分离病毒最常用的细胞系。用虹鳟体外基因组研究了15个病毒科。虹鳟细胞系最能支持疱疹病毒科、虹彩病毒科、双RNA病毒科、披膜病毒科和弹状病毒科病毒的复制。
