Grunow Bianka, Di Leonardo Valeria, Tönißen Katrin
Work Group "Fish Growth Physiology", Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
Toxics. 2025 Aug 20;13(8):696. doi: 10.3390/toxics13080696.
Advances in cell culture technology have led to fish cell lines being used as cost-effective, reproducible, and ethically favourable instruments in ecotoxicology. The development of new lines contributes to reducing animal experiments and improves model diversity. The brown trout (), an important bioindicator due to its sensitivity to pollutants, is still underrepresented in cell culture systems. In this study, two novel larvae-derived cell lines, STRlar1 and STRlar2, were established and maintained for over 40 passages. Although derived from sibling larvae of the same parental strain, the two lines showed marked differences in growth dynamics and toxicological responses to ethanol, dimethyl sulfoxide, isopropanol, and acetone. STRlar2 showed greater sensitivity to all chemicals tested, while STRlar1 exhibited longer proliferation and higher impedance, suggesting stronger substrate adhesion. These differences emphasise the importance of cellular heterogeneity, even in closely related lines. Our results underline the need to carefully validate new in vitro models and caution against relying on single cell lines. To improve the robustness and reliability of ecotoxicological assessments, we recommend the use of multiple independently derived lines that better reflect biological variability and reflect in vivo complexity.
细胞培养技术的进步使得鱼类细胞系成为生态毒理学中具有成本效益、可重复且符合伦理的工具。新细胞系的开发有助于减少动物实验并提高模型多样性。褐鳟因其对污染物敏感而成为重要的生物指标，但在细胞培养系统中的代表性仍然不足。在本研究中，建立了两个新的源自幼虫的细胞系STRlar1和STRlar2，并维持传代40多次。尽管这两个细胞系源自同一亲本菌株的同胞幼虫，但它们在生长动态以及对乙醇、二甲基亚砜、异丙醇和丙酮的毒理学反应方面表现出明显差异。STRlar2对所有测试化学物质表现出更高的敏感性，而STRlar1表现出更长的增殖时间和更高的阻抗，表明其对底物的粘附更强。这些差异强调了细胞异质性的重要性，即使在亲缘关系密切的细胞系中也是如此。我们的结果强调了仔细验证新的体外模型的必要性，并提醒不要依赖单一细胞系。为了提高生态毒理学评估的稳健性和可靠性，我们建议使用多个独立衍生的细胞系，以更好地反映生物变异性并体现体内复杂性。
