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.中MreCD的表征

Characterization of MreCD in .

作者信息

Chan Victor, Holcomb Tessa, Kaspar Justin R, Shields Robert C

机构信息

Department of Oral Biology, University of Florida, Gainesville, FL, USA.

Department of Biological Sciences, Arkansas State University, Jonesboro, AR, USA.

出版信息

J Oral Microbiol. 2025 Apr 4;17(1):2487643. doi: 10.1080/20002297.2025.2487643. eCollection 2025.

DOI:10.1080/20002297.2025.2487643
PMID:40206099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11980242/
Abstract

BACKGROUND

Activities that control cell shape and division are critical for the survival of bacteria. However, little is known about the circuitry controlling these processes in the dental caries pathogen .

METHODOLOGY

We designed experiments to characterize two genes, and , in Assays included cell morphology imaging, protein interaction analysis, transcriptomics, proteomics, and biofilm studies to generate a comprehensive understanding of the role of MreCD in .

RESULTS

Consistent with participating in cell elongation, cells lacking these genes were found to be rounder than wild-type cells. Using bacterial two-hybrid assays, interactions between MreCD and several other proteins implicated in cell elongation were observed. Further characterization, using proteomics, revealed that the surface-associated proteome is different in mutants lacking . Consistent with these changes we observed altered sucrose-mediated biofilm architecture. Loss of also had a noticeable impact on bacteriocin gene expression, which could account in part for the observation that mutants had a diminished capacity to compete with commensal streptococci.

CONCLUSION

Our results provide evidence that cell elongation proteins are required for normal physiology and establish a foundation for additional examination of these and related proteins in this organism.

摘要

背景

控制细胞形状和分裂的活动对细菌的生存至关重要。然而，对于龋齿病原体中控制这些过程的信号通路却知之甚少。

方法

我们设计了实验来表征在[具体细菌名称]中的两个基因[基因名称1]和[基因名称2]。实验包括细胞形态成像、蛋白质相互作用分析、转录组学、蛋白质组学和生物膜研究，以全面了解MreCD在[具体细菌名称]中的作用。

结果

与[具体细菌名称]参与细胞伸长一致，发现缺乏这些基因的细胞比野生型细胞更圆。使用细菌双杂交试验，观察到MreCD与其他几种参与细胞伸长的蛋白质之间的相互作用。通过蛋白质组学进一步表征发现，在缺乏[具体基因名称]的突变体中，表面相关蛋白质组有所不同。与这些变化一致，我们观察到蔗糖介导的生物膜结构发生改变。[具体基因名称]的缺失对细菌素基因表达也有显著影响，这可以部分解释[具体细菌名称]突变体与共生链球菌竞争能力减弱的现象。

结论

我们的结果提供了证据，表明细胞伸长蛋白是[具体细菌名称]正常生理所必需的，并为进一步研究该生物体中的这些及相关蛋白质奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/22108ad0b705/ZJOM_A_2487643_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/e37645a31954/ZJOM_A_2487643_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/3070c8fdb222/ZJOM_A_2487643_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/b8c22bc79674/ZJOM_A_2487643_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/2b8cf881f548/ZJOM_A_2487643_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/46ef1903cd67/ZJOM_A_2487643_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/22108ad0b705/ZJOM_A_2487643_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/e37645a31954/ZJOM_A_2487643_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/3070c8fdb222/ZJOM_A_2487643_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/b8c22bc79674/ZJOM_A_2487643_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/2b8cf881f548/ZJOM_A_2487643_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/46ef1903cd67/ZJOM_A_2487643_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4e/11980242/22108ad0b705/ZJOM_A_2487643_F0006_OC.jpg

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本文引用的文献

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Class A PBPs: It is time to rethink traditional paradigms.A 类青霉素结合蛋白：是时候重新思考传统的模式了。
Mol Microbiol. 2021 Jul;116(1):41-52. doi: 10.1111/mmi.14714. Epub 2021 Mar 23.
2
MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA.MreC 和 MreD 平衡延伸复合物蛋白 PBP2 和 RodA 之间的相互作用。
PLoS Genet. 2020 Dec 28;16(12):e1009276. doi: 10.1371/journal.pgen.1009276. eCollection 2020 Dec.
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A CozE Homolog Contributes to Cell Size Homeostasis of Streptococcus pneumoniae.CozE 同源物有助于肺炎链球菌的细胞大小稳态。
mBio. 2020 Oct 27;11(5):e02461-20. doi: 10.1128/mBio.02461-20.
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Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function.将变异链球菌 CRISPR-Cas9 系统重新用于理解必需基因功能。
PLoS Pathog. 2020 Mar 9;16(3):e1008344. doi: 10.1371/journal.ppat.1008344. eCollection 2020 Mar.
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Environmental Triggers of Expression in .环境触发因素在……中的表达
Front Microbiol. 2020 Jan 28;11:18. doi: 10.3389/fmicb.2020.00018. eCollection 2020.
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Characterization of LrgAB as a stationary phase-specific pyruvate uptake system in Streptococcus mutans.鉴定 LrgAB 在变形链球菌中作为一个特定于稳定期的丙酮酸摄取系统。
BMC Microbiol. 2019 Oct 12;19(1):223. doi: 10.1186/s12866-019-1600-x.
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Streptococcus mutans requires mature rhamnose-glucose polysaccharides for proper pathophysiology, morphogenesis and cellular division.变形链球菌需要成熟的鼠李糖-葡萄糖多糖才能正常进行病理生理学、形态发生和细胞分裂。
Mol Microbiol. 2019 Sep;112(3):944-959. doi: 10.1111/mmi.14330. Epub 2019 Jul 12.
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A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery.PBP2 在细菌细胞伸长机制激活肽聚糖聚合中起核心作用。
PLoS Genet. 2018 Oct 18;14(10):e1007726. doi: 10.1371/journal.pgen.1007726. eCollection 2018 Oct.
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Are the mutans streptococci still considered relevant to understanding the microbial etiology of dental caries?变形链球菌是否仍被认为与理解龋齿的微生物病因学相关？
BMC Oral Health. 2018 Jul 31;18(1):129. doi: 10.1186/s12903-018-0595-2.
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CozEa and CozEb play overlapping and essential roles in controlling cell division in Staphylococcus aureus.CozEa 和 CozEb 在金黄色葡萄球菌的细胞分裂控制中发挥重叠且必不可少的作用。
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