Kong Anne M, Liu Guei-Sheung, Mitchell Geraldine M, Lim Shiang Y
O'Brien Institute Department, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.
Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, VIC, Australia.
Methods Mol Biol. 2025;2922:241-248. doi: 10.1007/978-1-0716-4510-9_18.
The success of tissue engineering relies heavily on the efficient and rapid formation of blood vessels in the newly engineered tissues. To achieve this, it is crucial to use scaffolds that not only support the survival of vascular cells but also facilitate the development and maturation of a capillary network in vivo. Utilizing advanced biomaterials and scaffold designs is essential to ensure that the engineered tissues can integrate effectively with the host tissues and maintain their functionality over time. Here, we present a method for generating endothelial cells from induced pluripotent stem cells and pre-vascularizing scaffolds by seeding them with endothelial cells in a 3D porous structure. These pre-vascularized scaffolds can then be implanted in a rat subcutaneous model to create vascularized tissue constructs. This approach holds great promise for generating clinically viable constructs that can be used in regenerative medicine and drug development.
组织工程的成功在很大程度上依赖于新构建组织中血管的高效快速形成。要实现这一点，使用不仅能支持血管细胞存活，还能促进体内毛细血管网络发育和成熟的支架至关重要。利用先进的生物材料和支架设计对于确保工程组织能够与宿主组织有效整合并长期维持其功能至关重要。在此，我们提出一种从诱导多能干细胞生成内皮细胞并通过在三维多孔结构中接种内皮细胞来预血管化支架的方法。然后可以将这些预血管化支架植入大鼠皮下模型中以创建血管化组织构建体。这种方法对于生成可用于再生医学和药物开发的临床可行构建体具有巨大潜力。
