Yamamoto K, Hashimoto K, Chiba J, Simizu B
J Virol. 1985 Sep;55(3):840-2. doi: 10.1128/JVI.55.3.840-842.1985.
To analyze the biological activities of the alphavirus glycoproteins, eight different monoclonal antibodies against the two glycoproteins of western equine encephalitis virus were isolated. Five of the eight monoclonal antibodies were shown to be specific for E1 and three for E2 protein by an enzyme-linked immunosorbent assay and by radioimmunoprecipitation. Three of the five anti-E1 and all of the anti-E2 monoclonal antibodies inhibited hemagglutination by purified virions. One anti-E1 and two anti-E2 monoclonal antibodies possessed high virus-neutralizing activity.
为分析甲病毒糖蛋白的生物学活性，分离出了8种针对西部马脑炎病毒两种糖蛋白的不同单克隆抗体。通过酶联免疫吸附测定和放射免疫沉淀法显示，这8种单克隆抗体中有5种对E1蛋白具有特异性，3种对E2蛋白具有特异性。5种抗E1单克隆抗体中的3种以及所有抗E2单克隆抗体均抑制纯化病毒粒子的血凝作用。1种抗E1单克隆抗体和2种抗E2单克隆抗体具有高病毒中和活性。
