Zhang Yuyan, Guo Xin, Ma Ruijuan, Yu Mingliang, Xu Jianlan, Shen Zhijun
Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2025 Mar 27;16:1558619. doi: 10.3389/fpls.2025.1558619. eCollection 2025.
is an excellent woody species with high resistance to various abiotic and biotic stresses. The species is also a potential gene donor due to its important developmental prospects. However, the characterisation of the mitochondrial genome (mitogenome) remains unexplored. In this study, we sequenced and assembled the mitogenome of using reads from Illumina sequencing and the Oxford Nanopore platform. According to the results, the mitogenome is 407,608 bp in length, with a GC content of 45.5%. It contains 64 genes, including 39 protein-coding genes, 22 tRNA genes and 3 rRNA genes. Codon usage, repetitive sequences, nonsynonymous to synonymous substitution ratios, RNA editing, synteny, and phylogenetic relationships among Rosaceae species and gene migration events were investigated. In addition, two novel indel markers were developed to distinguish several species (i.e. , , and ). The results of our analyses provide valuable information and a theoretical basis for future research on .
是一种优良的木本物种，对各种非生物和生物胁迫具有高度抗性。由于其重要的发展前景，该物种也是一个潜在的基因供体。然而，线粒体基因组（mitogenome）的特征仍未被探索。在本研究中，我们使用来自Illumina测序和牛津纳米孔平台的读数对 的线粒体基因组进行了测序和组装。根据结果， 的线粒体基因组长度为407,608 bp，GC含量为45.5%。它包含64个基因，包括39个蛋白质编码基因、22个tRNA基因和3个rRNA基因。研究了蔷薇科物种之间的密码子使用、重复序列、非同义替换与同义替换比率、RNA编辑、共线性和系统发育关系以及基因迁移事件。此外，还开发了两个新的插入缺失标记来区分几个 物种（即 、 、 和 ）。我们的分析结果为未来对 的研究提供了有价值的信息和理论基础。
