Wolff R L, Combe N A, Entressangles B
Lipids. 1985 Jun;20(6):367-72. doi: 10.1007/BF02534204.
Effects of dietary trielaidin upon the alkenyl chain profile of plasmalogens were studied using heart mitochondria of rats fed a semi-purified diet containing 10% of fat supplement in which elaidic acid accounted for 69% of total fatty acids. Alkyl substituted dioxane (ASD) derivatives of the alkenyl groups of plasmalogens were prepared and analyzed by silver nitrate TLC and by GLC on different phases (BDS and OV-275). After two months of feeding the experimental diet, 40% of the ASD contained a trans-octadecenyl chain, suggesting that dietary elaidic acid was reduced in vivo to the corresponding alcohol and incorporated into plasmalogens. There was a simultaneous decrease in the percentage of ASD containing saturated chains, but the percentage of ASD substituted with cis-octadecenyl chains was not significantly affected. These observations suggested that elaidic acid may compete with saturated fatty acids, but not with cis-octadecenoic acids during the plasmalogen biosynthesis. Feeding trielaidin did not seem to have any significant influence on the relative proportions of plasmalogens, which accounted for 11-12%, on a phosphorus basis, of total heart mitochondria phospholipids.
使用喂食含10%脂肪补充剂的半纯化饮食的大鼠心脏线粒体，研究了膳食三油精对缩醛磷脂烯基链谱的影响，其中反油酸占总脂肪酸的69%。制备了缩醛磷脂烯基的烷基取代二恶烷（ASD）衍生物，并通过硝酸银薄层层析和在不同固定相（BDS和OV-275）上的气相色谱法进行分析。喂食实验饮食两个月后，40%的ASD含有反式十八碳烯基链，这表明膳食反油酸在体内被还原为相应的醇并掺入缩醛磷脂中。含有饱和链的ASD百分比同时下降，但被顺式十八碳烯基链取代的ASD百分比没有受到显著影响。这些观察结果表明，在缩醛磷脂生物合成过程中，反油酸可能与饱和脂肪酸竞争，但不与顺式十八碳烯酸竞争。喂食三油精似乎对缩醛磷脂的相对比例没有任何显著影响，以磷计，缩醛磷脂占心脏线粒体总磷脂的11-12%。
