Weber N, Richter I
Biochim Biophys Acta. 1982 May 13;711(2):197-207. doi: 10.1016/0005-2760(82)90027-3.
The incorporation of radioactivity from individual constituents of an equimolar mixture of saturated straight-chain alcohols (14:0, 16:0, 18:0, 20:0) and of nearly uniform mixtures of isomeric cis- or trans-octadecenols (delta 8-delta 16) into alkyl, alk-1-enyl and acyl moieties of diradylglycerophosphocholines and diradylglycerophosphoethanolamines and into alkyl and acyl moieties of wax esters was studied in rat brain as well as in L 1210 and S 180 ascites cells. The pattern of incorporation of radioactivity from the substrates into alkyl and alk-1-enyl moieties of ether phospholipids and into alkyl moieties of wax esters reveals the following: (1) The enzymes catalyzing the biosynthesis of alkylacylglycerols, the common intermediates of cholinephospholipids and ethanolaminephospholipids, have no substrate specificity with regard to position of the double bond of either cis- or trans-octadecenols or of intermediate ether lipids derived therefrom. (2) CDPcholine:diradylglycerol cholinephosphotransferases exhibit a strong preference for alkylacylglycerols with cis-8, cis-9 and cis-10-octadecenyl moieties, but no preference for the double bond position in the trans-octadecenylacylglycerols. (3) CDPethanolamine:diredylglycerol ethanolaminephosphotransferases have no substrate specificity with regard to position of the double bond in cis- or trans-octadecenyl moieties of alkylacylglycerols. (4) THe enzyme systems catalyzing the biosynthesis of alkylacylglycerophosphocholines and alkylacylglycerophosphoethanolamines exhibit substrate specificity with regard to chain-length of saturated alcohols and intermediate ether lipids derived therefrom. (5) Alkylacylglycerophosphoethanolamine desaturase and (6) wax ester synthase are highly specific for alkylacylglycerophosphoethanolamines and long-chain alcohols, respectively, with regard to chain-length of saturated alkyl moieties, but not with regard to position of double bonds of cis- or trans-octadecenyl moieties.
研究了饱和直链醇（14:0、16:0、18:0、20:0）等摩尔混合物以及异构顺式或反式十八碳烯醇（δ8 - δ16）近乎均匀混合物中的放射性成分掺入大鼠脑、L 1210和S 180腹水细胞中二烷基甘油磷酸胆碱和二烷基甘油磷酸乙醇胺的烷基、1-烯基和酰基部分以及蜡酯的烷基和酰基部分的情况。放射性从底物掺入醚磷脂的烷基和1-烯基部分以及蜡酯的烷基部分的模式显示如下：（1）催化烷基酰基甘油生物合成的酶，即胆碱磷脂和乙醇胺磷脂的常见中间体，对顺式或反式十八碳烯醇或由此衍生的中间醚脂质双键位置没有底物特异性。（2）CDP胆碱：二烷基甘油胆碱磷酸转移酶对具有顺式-8、顺式-9和顺式-10-十八碳烯基部分的烷基酰基甘油有强烈偏好，但对反式十八碳烯基酰基甘油中的双键位置没有偏好。（3）CDP乙醇胺：二烷基甘油乙醇胺磷酸转移酶对烷基酰基甘油顺式或反式十八碳烯基部分双键位置没有底物特异性。（4）催化烷基酰基甘油磷酸胆碱和烷基酰基甘油磷酸乙醇胺生物合成的酶系统对饱和醇及其衍生的中间醚脂质的链长表现出底物特异性。（5）烷基酰基甘油磷酸乙醇胺去饱和酶和（6）蜡酯合酶分别对饱和烷基部分的链长对烷基酰基甘油磷酸乙醇胺和长链醇具有高度特异性，但对顺式或反式十八碳烯基部分的双键位置没有特异性。
