Gregori Giulia, Mangoni Lorenzo, Muzi Alessio, Mogetta Veronica, Gujar Ramkailash, Chiapponi Mario, Fruttini Daniela, Dolz-Marco Rosa, Chhablani Jay, Mariotti Cesare, Lupidi Marco
Eye Clinic, Department of Experimental and Clinical Medicine, Polytechnic University of Marche, 60126 Ancona, Italy.
Humanitas Gradenigo Hospital, 10153 Torino, Italy.
J Clin Med. 2025 Mar 28;14(7):2312. doi: 10.3390/jcm14072312.
: The aim of this study was to quantify the interscan variability of vascular perfusion in patients with diabetic retinopathy (DR) using sequential optical coherence tomography angiography (OCT-A) scans. : Patients with low-grade DR (DRSS score 20) underwent five sequential OCT-A imaging sessions. Vessel perfusion density (VPD) values of the superficial capillary plexus (SCP), intermediate capillary plexus (ICP), deep capillary plexus (DCP), and choriocapillaris (CC) were computed using ImageJ Version 1.54 software via a binarization process. The binarized images were analyzed to compute VPD using vessel density plugins. Inter-session variability was assessed using the coefficient of variation (CV) and intraclass correlation coefficient (ICC). The results were compared with an age-matched group of healthy controls. : A total of 16 eyes from 16 patients with DR (6 females, 37.5%) and 16 eyes from healthy controls (6 females, 37.5%) were included. Mean VPD values for SCP, ICP, DCP, and CC in patients with DR were 23.64, 13.64, 15.31, and 36.57, respectively. Corresponding values for the healthy subjects were 37.65, 23.32, 22.05, and 49.00, respectively. No significant differences were found in VPD across consecutive scanning sessions ( > 0.05) in either group. However, significant differences in VPD were observed between patients who were healthy and patients with diabetes. : Sequential OCT-A imaging demonstrated no significant inter-session variability in both healthy subjects and subjects with diabetes. The Spectralis OCT-A system showed high reliability for imaging both healthy and diseased conditions.
本研究的目的是使用连续光学相干断层扫描血管造影（OCT-A）扫描来量化糖尿病视网膜病变（DR）患者血管灌注的扫描间变异性。患有轻度DR（糖尿病视网膜病变严重程度评分20）的患者接受了五次连续的OCT-A成像检查。使用ImageJ 1.54版本软件通过二值化过程计算浅表毛细血管丛（SCP）、中间毛细血管丛（ICP）、深层毛细血管丛（DCP）和脉络膜毛细血管（CC）的血管灌注密度（VPD）值。使用血管密度插件对二值化图像进行分析以计算VPD。使用变异系数（CV）和组内相关系数（ICC）评估检查间变异性。将结果与年龄匹配的健康对照组进行比较。共纳入了16例DR患者的16只眼（6例女性，37.5%）和健康对照组的16只眼（6例女性，37.5%）。DR患者中SCP、ICP、DCP和CC的平均VPD值分别为23.64、13.64、15.31和36.57。健康受试者的相应值分别为37.65、23.32、22.05和49.00。两组中连续扫描检查间的VPD均未发现显著差异（>0.05）。然而，健康患者和糖尿病患者之间的VPD存在显著差异。连续OCT-A成像显示健康受试者和糖尿病受试者的检查间均无显著变异性。Spectralis OCT-A系统在健康和患病情况下的成像均显示出高可靠性。
