Chevallier Pascale, Wiggers Helton José, Copes Francesco, Zorzi Bueno Cecilia, Mantovani Diego
Laboratory for Biomaterials and Bioengineering (LBB-UL), Canada Research Chair Tier I, Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Division Regenerative Medicine, Laval University, Quebec City, QC G1V0A6, Canada.
Laboratory for Biomaterials and Bioengineering (LBB-BPK), Associação de Ensino, Pesquisa e Extensão BIOPARK, Max Planck Avenue, 3797, Building Charles Darwin, Toledo 85919-899, PR, Brazil.
Nanomaterials (Basel). 2023 Jan 25;13(3):484. doi: 10.3390/nano13030484.
Healthcare-associated infections (HAIs) represent a global burden, leading to significant mortality and generating financial costs. One important cause of HAIs is the microbiological contamination of implantable medical devices. In this context, a novel antimicrobial drug-eluting system, based on chitosan and loaded with gentamicin, a broad-spectrum antibiotic, was developed. The effects of the addition of tannic acid and different FeSO concentrations on the loaded antibiotic release were evaluated. The properties of the films were assessed in terms of thickness, swelling, mass loss and wettability. The films' surface composition was characterized by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy. The antibiotic release in phosphate buffer saline was quantified by high-performance liquid chromatography-mass spectrometry, and the antibacterial activity was evaluated. Hemolysis and cytotoxicity were also assessed. The results showed that the addition of tannic acid and iron decreased the swelling degree and degradation due to strong interactions between the different components, thus impacting gentamicin release for up to 35 days. In conclusion, this study presents a novel strategy to produce low-cost and biocompatible antimicrobial drug-eluting systems with sustained and prolonged antibacterial activity over more than a month.
医疗保健相关感染（HAIs）是一项全球性负担，会导致大量死亡并产生经济成本。HAIs的一个重要原因是可植入医疗设备的微生物污染。在此背景下，开发了一种基于壳聚糖并负载有广谱抗生素庆大霉素的新型抗菌药物洗脱系统。评估了添加单宁酸和不同浓度硫酸亚铁对负载抗生素释放的影响。从厚度、溶胀、质量损失和润湿性方面评估了薄膜的性能。通过X射线光电子能谱和傅里叶变换红外光谱对薄膜的表面成分进行了表征。通过高效液相色谱-质谱法定量磷酸盐缓冲盐水中的抗生素释放，并评估抗菌活性。还评估了溶血和细胞毒性。结果表明，由于不同成分之间的强烈相互作用，单宁酸和铁的添加降低了溶胀程度和降解，从而影响庆大霉素释放长达35天。总之，本研究提出了一种新策略，可生产具有低成本和生物相容性的抗菌药物洗脱系统，其抗菌活性可持续长达一个多月。
