Oinam Lalhaba, Minoshima Fumi, Tateno Hiroaki
Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
AMED-Prime, AMED, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
ISME Commun. 2022 Jan 5;2(1):1. doi: 10.1038/s43705-021-00084-2.
Bacterial glycans modulate the cross talk between the gut microbiota and its host. However, little is known about these glycans because of the lack of appropriate technology to study them. In this study, we applied Glycan-seq technology for glycan profiling of the intact gut microbiota of mice. The evaluation of cultured gram-positive (Deinococcus radiodurans) and gram-negative (Escherichia coli) bacteria showed significantly distinct glycan profiles between these bacteria, which were selected and further analyzed by flow cytometry. The results of flow cytometry agreed well with those obtained by Glycan-seq, indicating that Glycan-seq can be used for bacterial glycan profiling. We thus applied Glycan-seq for comparative glycan profiling of pups and adult mice gut microbiotas. The glycans of the pups and adult microbiotas had significantly distinct glycan profiles, which reflect the different bacterial compositions of pups and adult gut microbiotas based on 16S rRNA gene sequencing.α2-6Sia-binders bound specifically to the pups microbiota. Lectin pull-down followed by 16S rRNA gene sequencing of the pups microbiota identified Lactobacillaceae as the most abundant bacterial family with glycans reacting with α2-6Sia-binders. The Glycan-seq system can reveal the glycan profile of the intact bacterial gut microbiota.
细菌聚糖可调节肠道微生物群与其宿主之间的相互作用。然而，由于缺乏研究这些聚糖的合适技术，人们对它们了解甚少。在本研究中，我们应用聚糖测序（Glycan-seq）技术对小鼠完整肠道微生物群进行聚糖谱分析。对培养的革兰氏阳性菌（耐辐射球菌）和革兰氏阴性菌（大肠杆菌）的评估显示，这些细菌之间的聚糖谱存在显著差异，我们通过流式细胞术对其进行了筛选和进一步分析。流式细胞术的结果与聚糖测序（Glycan-seq）获得的结果高度一致，表明聚糖测序（Glycan-seq）可用于细菌聚糖谱分析。因此，我们应用聚糖测序（Glycan-seq）对幼鼠和成鼠的肠道微生物群进行比较聚糖谱分析。幼鼠和成鼠微生物群的聚糖具有显著不同的聚糖谱，这反映了基于16S rRNA基因测序的幼鼠和成鼠肠道微生物群不同的细菌组成。α2-6唾液酸结合剂与幼鼠微生物群特异性结合。凝集素下拉结合幼鼠微生物群的16S rRNA基因测序确定，乳酸杆菌科是聚糖与α2-6唾液酸结合剂反应的最丰富细菌家族。聚糖测序（Glycan-seq）系统可以揭示完整细菌肠道微生物群的聚糖谱。
