类风湿关节炎合并乙型肝炎病毒感染患者外周血淋巴细胞主要亚群的特征：一项回顾性队列研究
The characteristics of peripheral blood lymphocyte main subsets in rheumatoid arthritis patients concurrent with hepatitis b virus infection: a retrospective cohort study.
作者信息
Shao Lijia, Shen Lihong, Wu Junqi
机构信息
Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China.
出版信息
BMC Infect Dis. 2025 Apr 12;25(1):517. doi: 10.1186/s12879-025-10932-4.
OBJECTIVE
The distribution of peripheral blood lymphocytes may change due to the influence of diseases. This study aims to investigate the impact of Hepatitis B virus (HBV) infection on the distribution of peripheral blood lymphocyte subsets in patients with Rheumatoid Arthritis (RA).
METHODS
Two hundred ninety-eight patients were recruited from a retrospective cohort of patients with RA. Patients with RA (n = 43) who had hepatitis B surface antigen (HBsAg) positivity in the serum were categorized into the HBV group (HBV-RA group), while 255 RA patients without HBsAg positivity were assigned to the control group. The patients in the HBV-RA group were further divided into two subgroups based on their levels of HBV DNA: those with levels below the lower limit of quantification (< 20 IU/ml) formed the HBV DNA group, while those with levels above or equal to this limit (≥ 20 IU/ml) constituted the HBV DNA group. Demographic, clinical and laboratory data were also collected.
RESULTS
Compared with those of the control group, a higher proportion of CD19 B cells and CD8 T cells and a lower CD4/CD8 ratio were observed in the HBV-RA group (all P < 0.05). The same trend was observed in the HBV DNA group compared to the HBV DNA group (all P < 0.05). Additionally, based on multivariable logistic regression analysis, the male gender, DAS-28 ≥ 2.6, a high proportion of CD19 + B and CD8 + T cells, and the elevated levels of rheumatoid factor (RF) were found to be significantly associated with RA concurrent with HBV infection (all P < 0.05). In the HBV-RA group, a correlation analysis was conducted revealing a positive association between CD19 + B cells and DAS-28 score, as well as CD8 + T cells and DAS-28 score. There was no statistically significant difference in CD4/CD8 ratio between different DAS-28 groups, however the study revealed a significant negative association between the ratio of CD4 +/CD8 + and the DAS-28 score.
CONCLUSION
The high proportion of CD19 + B and CD8 + T cells were closely associated with RA concurrent with HBV infection.
目的
疾病的影响可能会导致外周血淋巴细胞的分布发生变化。本研究旨在探讨乙型肝炎病毒（HBV）感染对类风湿关节炎（RA）患者外周血淋巴细胞亚群分布的影响。
方法
从RA患者的回顾性队列中招募了298例患者。血清中乙型肝炎表面抗原（HBsAg）呈阳性的RA患者（n = 43）被归类为HBV组（HBV-RA组），而255例无HBsAg阳性的RA患者被分配到对照组。HBV-RA组的患者根据其HBV DNA水平进一步分为两个亚组：水平低于定量下限（<20 IU/ml）的患者组成HBV DNA阴性组，而水平高于或等于此限值（≥20 IU/ml）的患者构成HBV DNA阳性组。还收集了人口统计学、临床和实验室数据。
结果
与对照组相比，HBV-RA组中CD19+B细胞和CD8+T细胞的比例更高，CD4/CD8比值更低（所有P<0.05）。与HBV DNA阴性组相比，HBV DNA阳性组也观察到相同的趋势（所有P<0.05）。此外，基于多变量逻辑回归分析，发现男性、DAS-28≥2.6、CD19+B和CD8+T细胞比例高以及类风湿因子（RF）水平升高与RA合并HBV感染显著相关（所有P<0.05）。在HBV-RA组中，进行了相关性分析，结果显示CD19+B细胞与DAS-28评分之间以及CD8+T细胞与DAS-28评分之间呈正相关。不同DAS-28组之间的CD4/CD8比值没有统计学上的显著差异，然而该研究显示CD4+/CD8+比值与DAS-28评分之间存在显著负相关。
结论
CD19+B和CD8+T细胞比例高与RA合并HBV感染密切相关。
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