实验性溃疡性结肠炎骨髓间充质干细胞移植的最佳剂量

Optimal dose of bone marrow mesenchymal stem cell transplantation for experimental ulcerative colitis.

作者信息

Chen Xiaoyun, Xia Yan, Min Min, Qin Lingzhi, Liu Yangsheng

机构信息

Department of Pathology, Wuhan No.1 Hospital, Wuhan, 430030, China.

School of Biomedical Engineering and Medical Imaging, Xianning Medical College, Hubei University of Science and Technology, Xianning, 437100, China.

出版信息

Regen Ther. 2025 Mar 26;29:177-183. doi: 10.1016/j.reth.2025.01.015. eCollection 2025 Jun.

DOI:10.1016/j.reth.2025.01.015

PMID:40225050

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11986536/

Abstract

OBJECTIVE

To investigate the optimal dose of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat.

METHODS

The BMSC of SD rat were isolated, cultured and labelled with DAPI. SD rats were randomly distributed into 3 groups, Colitis was induced with immune-combined TNBS/ethanol in group A、B、C, 3 groups received caudal vein injection of 1 mL fluids, which contain cell number 1 × 10、5 × 10、1 × 10 separately. 5 rats in each group were sacrificed at day 7 and 14 after injection, Cryostat sections of gut, The number of BMSCs in colon and normal tissue surrounded was observed with fluorescent microscope.

RESULTS

The DAPI marked BMSCs could been seen in the colic mucosa in each group on day 7、14, more cells in colon than the surrounding normal tissue, compared with 1 × 10 group, More cells in 5 × 10 group (P < 0.05), there were no significant difference (P > 0.05) between 5 × 10 group and 1 × 10 group. There were more cells in colon on 14 day than 7 day, and less in the surrounding normal tissue on 14 day than 7 day.

CONCLUSIONS

The density 5 × 10 is proper of bone mesenchymal stem cells for treatment of ulcerative colitis.

摘要

目的

探讨骨髓间充质干细胞移植治疗溃疡性结肠炎大鼠的最佳剂量。

方法

分离、培养SD大鼠的骨髓间充质干细胞并用DAPI标记。将SD大鼠随机分为3组，A、B、C组用免疫联合TNBS/乙醇诱导结肠炎，3组分别经尾静脉注射1 mL含细胞数为1×10、5×10、1×10的液体。注射后第7天和第14天处死每组5只大鼠，取肠道冰冻切片，用荧光显微镜观察结肠及周围正常组织中骨髓间充质干细胞的数量。