• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

自噬缺陷型出芽酵母细胞对冻融应激敏感。

Autophagy-deficient budding yeast cells are sensitive to freeze-thaw stress.

作者信息

James Maria, Klain Grace K, Brito Stacey O, Trejo Lupita, Okello Teresa M A, Segarra Verónica A

机构信息

Department of Biological Sciences, Goucher College, Towson, Maryland, United States.

Department of Chemistry, Goucher College, Towson, Maryland, United States.

出版信息

MicroPubl Biol. 2025 Mar 27;2025. doi: 10.17912/micropub.biology.000929. eCollection 2025.

DOI:10.17912/micropub.biology.000929
PMID:40226511
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11986705/
Abstract

Autophagy enables eukaryotes to recycle damaged and unneeded materials to ensure survival in times of stress such as starvation. However, the full range of cellular stress responses that activate and require autophagy remains unknown. This study has compared the survival of wild type, and budding yeast cells following freeze-thaw stress. The results indicate that cells deficient in autophagy exhibit enhanced sensitivity to freeze-thaw stress.

摘要

自噬使真核生物能够回收受损和不需要的物质，以确保在饥饿等应激条件下存活。然而，激活并需要自噬的细胞应激反应的全貌仍不清楚。本研究比较了野生型和芽殖酵母细胞在冻融应激后的存活率。结果表明，自噬缺陷的细胞对冻融应激表现出更高的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec13/11986705/2a7591fb763b/25789430-2025-micropub.biology.000929.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec13/11986705/2a7591fb763b/25789430-2025-micropub.biology.000929.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec13/11986705/2a7591fb763b/25789430-2025-micropub.biology.000929.jpg

相似文献

1
Autophagy-deficient budding yeast cells are sensitive to freeze-thaw stress.自噬缺陷型出芽酵母细胞对冻融应激敏感。
MicroPubl Biol. 2025 Mar 27;2025. doi: 10.17912/micropub.biology.000929. eCollection 2025.
2
Importance of Proteasome Gene Expression during Model Dough Fermentation after Preservation of Baker's Yeast Cells by Freezing.在冷冻保存面包酵母细胞后，通过模型面团发酵研究蛋白酶体基因表达的重要性。
Appl Environ Microbiol. 2018 May 31;84(12). doi: 10.1128/AEM.00406-18. Print 2018 Jun 15.
3
is conserved between and psychrophilic, polar-collected fungi.在嗜冷的、从极地采集的真菌之间是保守的。
MicroPubl Biol. 2021 Aug 16;2021. doi: 10.17912/micropub.biology.000446. eCollection 2021.
4
The freeze-thaw stress response of the yeast Saccharomyces cerevisiae is growth phase specific and is controlled by nutritional state via the RAS-cyclic AMP signal transduction pathway.酿酒酵母的冻融应激反应具有生长阶段特异性，并通过RAS-环磷酸腺苷信号转导途径受营养状态的控制。
Appl Environ Microbiol. 1997 Oct;63(10):3818-24. doi: 10.1128/aem.63.10.3818-3824.1997.
5
Cryopreservation and the Freeze-Thaw Stress Response in Yeast.酵母的冷冻保存和冻融应激反应。
Genes (Basel). 2020 Jul 22;11(8):835. doi: 10.3390/genes11080835.
6
Systematic Characterization and Analysis of the Freeze-Thaw Tolerance Gene Set in the Budding Yeast, .芽殖酵母中冻融耐受性基因集的系统表征与分析
Int J Mol Sci. 2025 Feb 27;26(5):2149. doi: 10.3390/ijms26052149.
7
Metabolomics analysis of freeze-thaw tolerance enhancement mechanism of ε-poly-l-lysine on industrial yeast.工业酵母中ε-聚赖氨酸提高抗冻能力的代谢组学分析。
Food Chem. 2022 Jul 15;382:132315. doi: 10.1016/j.foodchem.2022.132315. Epub 2022 Feb 2.
8
Enhancing freeze-thaw tolerance in baker's yeast: strategies and perspectives.提高面包酵母的冻融耐受性：策略与展望
Food Sci Biotechnol. 2024 Jul 3;33(13):2953-2969. doi: 10.1007/s10068-024-01637-6. eCollection 2024 Oct.
9
Intracellular trehalose accumulation via the Agt1 transporter promotes freeze-thaw tolerance in Saccharomyces cerevisiae.通过 Agt1 转运蛋白积累细胞内海藻糖可提高酿酒酵母的抗冻-解冻能力。
J Appl Microbiol. 2022 Oct;133(4):2390-2402. doi: 10.1111/jam.15700. Epub 2022 Jul 20.
10
Hsp104 contributes to freeze-thaw tolerance by maintaining proteasomal activity in a spore clone isolated from Shirakami kodama yeast.Hsp104 通过维持来自 Shirakami kodama 酵母的孢子克隆中的蛋白酶体活性来促进抗冻-解冻耐受性。
J Gen Appl Microbiol. 2021 Oct 22;67(4):170-178. doi: 10.2323/jgam.2020.11.002. Epub 2021 Jun 19.

本文引用的文献

1
Intracellular trehalose accumulation via the Agt1 transporter promotes freeze-thaw tolerance in Saccharomyces cerevisiae.通过 Agt1 转运蛋白积累细胞内海藻糖可提高酿酒酵母的抗冻-解冻能力。
J Appl Microbiol. 2022 Oct;133(4):2390-2402. doi: 10.1111/jam.15700. Epub 2022 Jul 20.
2
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants.线粒体融合形成三维网络、自噬以及增加细胞器接触是植物冷胁迫过程中的重要亚细胞特征。
Int J Mol Sci. 2020 Nov 19;21(22):8753. doi: 10.3390/ijms21228753.
3
Cryopreservation and the Freeze-Thaw Stress Response in Yeast.
酵母的冷冻保存和冻融应激反应。
Genes (Basel). 2020 Jul 22;11(8):835. doi: 10.3390/genes11080835.
4
Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay.ATG5基因的突变会降低自噬水平，并导致伴有发育迟缓的共济失调。
Elife. 2016 Jan 26;5:e12245. doi: 10.7554/eLife.12245.
5
Trypan Blue Exclusion Test of Cell Viability.细胞活力的台盼蓝排斥试验
Curr Protoc Immunol. 2015 Nov 2;111:A3.B.1-A3.B.3. doi: 10.1002/0471142735.ima03bs111.
6
Why is intracellular ice lethal? A microscopical study showing evidence of programmed cell death in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum.为什么细胞内结冰是致命的？一项显微镜研究显示了在低温处理的糖槭顽拗性种子胚轴中存在程序性细胞死亡的证据。
Ann Bot. 2015 May;115(6):991-1000. doi: 10.1093/aob/mcv009. Epub 2015 Mar 25.
7
Atg27 tyrosine sorting motif is important for its trafficking and Atg9 localization.自噬相关蛋白27（Atg27）的酪氨酸分选基序对其运输及自噬相关蛋白9（Atg9）的定位很重要。
Traffic. 2015 Apr;16(4):365-78. doi: 10.1111/tra.12253. Epub 2015 Feb 20.
8
Autophagy and the integrated stress response.自噬和综合应激反应。
Mol Cell. 2010 Oct 22;40(2):280-93. doi: 10.1016/j.molcel.2010.09.023.
9
Functional profiling of the Saccharomyces cerevisiae genome.酿酒酵母基因组的功能分析。
Nature. 2002 Jul 25;418(6896):387-91. doi: 10.1038/nature00935.
10
A protein conjugation system essential for autophagy.一种自噬所必需的蛋白质偶联系统。
Nature. 1998 Sep 24;395(6700):395-8. doi: 10.1038/26506.