Sewade Wilfrid, Polat Ceylan, Kasap Ozge Erisoz
Department of Biology, VERG Laboratories, Faculty of Science, Hacettepe University, Ankara, Türkiye.
Graduate School of Science and Engineering, Hacettepe University, Ankara, Türkiye.
Trop Med Int Health. 2025 Jun;30(6):498-508. doi: 10.1111/tmi.14099. Epub 2025 Apr 14.
Mosquitoes transmit various pathogens causing diseases like Zika, Dengue, West Nile and Chikungunya. They also harbour insect-specific viruses (ISVs) and Wolbachia, which can block arbovirus transmission. This study investigated the prevalence of Orthoflavivirus and Wolbachia in mosquito populations from three provinces in Türkiye.
Mosquitoes were collected using CDC Miniature Light traps in 2022-2023. Morphologically identified specimens were pooled (1-10 individuals) and screened for Orthoflavivirus and Wolbachia via PCR and confirmed by Sanger sequencing. Infection prevalence was estimated using the maximum likelihood method. Mosquito taxa richness across provinces was estimated using the abundance-based, non-parametric Chao1 index.
Among 8766 mosquitoes (11 taxa) collected, Culex perexiguus, Ochlerotatus caspius and Anopheles claviger were most abundant. Anopheles flavivirus (AnFV) detected in one Oc. caspius pool, while Wolbachia sequences belonging to supergroup B were detected in An. claviger, Cx. pipiens s.l., Cx. perexiguus and Oc. caspius, with an overall infection prevalence of 0.0119 (95% CI: 0.008-0.0161). The richest mosquito fauna was detected in Ankara, followed by Adana, and Çankırı.
This study provides new insights into mosquito richness and the prevalence of Orthoflavivirus and Wolbachia in Türkiye, contributing to vector surveillance and the potential use of Wolbachia in mosquito control strategies.
蚊子传播多种病原体，可引发寨卡、登革热、西尼罗河和基孔肯雅等疾病。它们还携带昆虫特异性病毒（ISV）和沃尔巴克氏体，后者可阻断虫媒病毒传播。本研究调查了土耳其三个省份蚊子种群中正黄病毒属和沃尔巴克氏体的流行情况。
于2022年至2023年使用疾控中心微型诱蚊灯诱捕蚊子。对形态学鉴定的标本进行合并（1至10只个体），通过聚合酶链反应（PCR）筛查正黄病毒属和沃尔巴克氏体，并通过桑格测序进行确认。使用最大似然法估计感染率。使用基于丰度的非参数Chao1指数估计各省蚊子分类群丰富度。
在收集的8766只蚊子（11个分类群）中，淡色库蚊、里海伊蚊和叉形按蚊最为常见。在一个里海伊蚊样本池中检测到按蚊黄病毒（AnFV），而在叉形按蚊、致倦库蚊复合组、淡色库蚊、里海伊蚊中检测到属于B超群的沃尔巴克氏体序列，总体感染率为0.0119（95%置信区间：0.008 - 0.0161）。在安卡拉检测到最丰富的蚊子种类，其次是阿达纳和昌克勒。
本研究为土耳其蚊子丰富度以及正黄病毒属和沃尔巴克氏体的流行情况提供了新见解，有助于病媒监测以及沃尔巴克氏体在蚊虫控制策略中的潜在应用。
