Yang Bohan, Li Chen, Li Rui, Peng Yachun, Li Xiangyi, Dong Zhigang, Zhao Qifeng, Lin James, Gao Qifei, Lu Jiang, Fu Peining
Center for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Network and Information Center, Shanghai Jiao Tong University, Shanghai, China.
Sci Data. 2025 Apr 14;12(1):619. doi: 10.1038/s41597-025-04765-8.
Grapevine is an important commercial crop widely grown in the world. However, grapevine cultivation has long been seriously threatened by grapevine downy mildew caused by Plasmopara viticola. Screening the resistance resources and identifying the resistance germplasm is the key to improve the resistance of cultivars to P. viticola. East Asia is one of the three origin regions of wild grapevine (North America, Eurasia and East Asia), and many varieties have strong disease resistance. In present study, we selected nine wild East Asian grape vines and selected three of them with the highest P. viticola resistance to construct genetic populations. Genotyping by Targeted Sequencing (GBTS) is a high-throughput and cost-effective technology for sequencing specific genome regions, and its application in genetic population analysis and disease resistance related markers identification has revealed that may be abundant P. viticola-resistant germplasm in East Asian grapes. Our sequencing data and disease resistance grade data provided a valuable reference for mining downy mildew resistant germplasm of East Asian grape and breeding resistant varieties.
葡萄是一种重要的经济作物，在全球广泛种植。然而，葡萄栽培长期以来一直受到由葡萄生单轴霉引起的葡萄霜霉病的严重威胁。筛选抗性资源并鉴定抗性种质是提高品种对葡萄生单轴霉抗性的关键。东亚是野生葡萄的三个起源地区之一（北美、欧亚大陆和东亚），许多品种具有很强的抗病性。在本研究中，我们选择了9种东亚野生葡萄，并挑选了其中对葡萄生单轴霉抗性最高的3种来构建遗传群体。靶向测序基因分型（GBTS）是一种用于对特定基因组区域进行测序的高通量且经济高效的技术，其在遗传群体分析和抗病相关标记鉴定中的应用表明东亚葡萄中可能存在丰富的抗葡萄生单轴霉种质。我们的测序数据和抗病等级数据为挖掘东亚葡萄抗霜霉病种质和培育抗病品种提供了有价值的参考。
