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单链 DNA 结合基因 () 具有来自 的强病原体诱导启动子,可增强对 的抗性。

The Single-Stranded DNA-Binding Gene () with a Strong Pathogen-Induced Promoter from Enhances Resistance to .

机构信息

Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.

Institute of Agricultural Bio-Resources Research, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.

出版信息

Int J Mol Sci. 2022 Jul 21;23(14):8052. doi: 10.3390/ijms23148052.

Abstract

plants are disease-susceptible while plants are disease-resistant; however, the molecular mechanism remains unclear. In this study, the single-stranded DNA- and RNA-binding protein gene ( and ) were cloned from "Cabernet Sauvignon" and "HD1". and promoter sequences ( and ) were also isolated; however, the identity of the promoter sequences was far lower than that between the coding sequences (CDSs). Both gene sequences had seven exons and six introns, and they had a C-terminal Whirly conserved domain and N-terminal chloroplast transit peptide, which was then verified to be chloroplast localization. Transcriptional expression showed that was strongly induced by , while showed a low expression level. Further, the GUS activity indicated had high activity involved in response to infection. In addition, transiently expressing and enhanced the resistance. Moreover, , and were upregulated in transgenic leaves. This research presented a novel insight into disease resistance mechanism that promoted the transcription of , which subsequently regulated the expression of and , further enhancing the resistance to .

摘要

植物易患病,而植物具有抗病性;然而,其分子机制尚不清楚。本研究从“赤霞珠”和“HD1”中克隆出单链 DNA 和 RNA 结合蛋白基因(和)。还分离了和启动子序列(和);然而,启动子序列的同一性远低于编码序列(CDS)之间的同一性。两个基因序列都有七个外显子和六个内含子,它们具有 C 端 Whirly 保守结构域和 N 端叶绿体转运肽,随后被验证为叶绿体定位。转录表达表明,被强烈诱导,而表现出低表达水平。此外,GUS 活性表明具有参与对感染反应的高活性。此外,瞬时表达和增强了抗性。此外,在转基因叶片中上调了、和。本研究为抗病机制提供了新的见解,即促进了的转录,进而调节了和的表达,从而增强了对的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d90/9315732/c1d709c9f540/ijms-23-08052-g001.jpg

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