Goodman W G
Proc Soc Exp Biol Med. 1985 Sep;179(4):509-16. doi: 10.3181/00379727-179-42131.
Osteomalacia has been noted following in vivo aluminum (Al) loading in the rat by some investigators but not by others. To determine whether the response of bone to Al differs as a function of the skeletal site examined, quantitative histology of cortical and trabecular bone was done in the tibiae from control (C, n = 10), Al-treated (AL, n = 9), nephrectomized control (NX-C, n = 7), and nephrectomized Al-treated (NX-AL, n = 8) rats given 2 mg/day of Al for 4 weeks. Bone Al content was determined by histochemical methods. In cortical bone, osteoid seam width, osteoid volume, and percent osteoid area were similar for all groups. In contrast, for trabecular bone, both forming surface (means +/- SD) (5.2 +/- 3.4 vs 1.8 +/- 1.1%, P less than 0.05) and osteoid volume (1.7 +/- 0.7 vs 1.0 +/- 0.4%, P less than 0.05) increased from control values in AL, although osteoid seam width did not differ. In NX-AL, trabecular forming surface (20.2 +/- 6.7 vs 6.2 +/- 2.4%, P less than 0.01), osteoid area (13.2 +/- 5.7 vs 3.5 +/- 0.8%, P less than 0.01), and osteoid width (18.7 +/- 5.7 vs 9.7 +/- 2.3 micron, P less than 0.01) all were greater than in NX-C. Deposits of Al were undetectable in C and NX-C, were minimal in cortical bone in AL and NX-AL, but were present at 40.5 +/- 11.5 and 71.1 +/ 6.5% of trabecular surfaces in AL and NX-AL, respectively. Osteoid area and osteoid surface each correlated with trabecular bone Al. Thus, (a) osteoid accumulates in trabecular, but not in cortical, bone after 4 weeks of Al loading; (b) the extent of osteoid accumulation correlates with the bone Al content; and (c) the histologic response to Al in cortical and trabecular bone is related to local differences in the uptake of Al into bone.
一些研究者注意到大鼠体内铝（Al）负荷后会出现骨软化症，而另一些研究者则未观察到。为了确定骨骼对铝的反应是否因所检查的骨骼部位不同而有所差异，对给予2毫克/天铝持续4周的对照（C，n = 10）、铝处理（AL，n = 9）、肾切除对照（NX - C，n = 7）和肾切除铝处理（NX - AL，n = 8）大鼠的胫骨皮质骨和小梁骨进行了定量组织学研究。通过组织化学方法测定骨铝含量。在皮质骨中，所有组的类骨质缝宽度、类骨质体积和类骨质面积百分比相似。相比之下，对于小梁骨，铝处理组的形成表面（平均值±标准差）（5.2±3.4对1.8±1.1%，P<0.05）和类骨质体积（1.7±0.7对1.0±0.4%，P<0.05）均较对照组增加，尽管类骨质缝宽度无差异。在肾切除铝处理组中，小梁形成表面（20.2±6.7对6.2±2.4%，P<0.01）、类骨质面积（13.2±5.7对3.5±0.8%，P<0.01）和类骨质宽度（18.7±5.7对9.7±2.3微米，P<0.01）均大于肾切除对照组。在对照和肾切除对照组中未检测到铝沉积，在铝处理组和肾切除铝处理组的皮质骨中铝沉积最少，但分别在铝处理组和肾切除铝处理组的小梁表面的40.5±11.5%和71.1±6.5%处存在铝沉积。类骨质面积和类骨质表面均与小梁骨铝相关。因此，（a）铝负荷4周后，类骨质在小梁骨而非皮质骨中蓄积；（b）类骨质蓄积程度与骨铝含量相关；（c）皮质骨和小梁骨对铝的组织学反应与铝在骨中的摄取局部差异有关。
