Goodman W G, Henry D A, Horst R, Nudelman R K, Alfrey A C, Coburn J W
Kidney Int. 1984 Feb;25(2):370-5. doi: 10.1038/ki.1984.26.
There is an association between bone aluminum (Al) accumulation and dialysis-associated osteomalacia (OM). To study whether Al is pathogenic in OM, quantitative bone histomorphometry was done in six dogs before (Bx 1) and after (Bx 2) 3 to 5 weeks of intravenous Al administration (1 mg Al /kg/day). Bone Al was determined by histochemical and chemical methods. The percent osteoid rose from 2.8 +/- 0.8 to 7.0 +/- 4.3% (mean +/- SD), P less than 0.05, and osteoid width increased from 5.7 +/- 0.6 to 8.0 +/- 1.2 mu, P less than 0.01, after Al. Bone Al rose from 1.3 +/- 1.6 to 94.0 +/- 19.0 mg/kg after Al, and the severity of OM, expressed as either percent forming surface or percent osteoid, correlated with bone Al measured histochemically and expressed as either percent surface or percent area of trabecular bone staining for Al (r = 0.85 - 0.90, P less than 0.01). Poor tetracycline uptake (six dogs), which indicates impaired mineralization, and little or no separation of tetracycline labels (four dogs) were noted at Bx 2; thus, bone apposition and formation rates were below the limits of detection. Resorptive surface did not change but trabecular volume, expressed as percent of tissue volume, fell from 22.1 +/- 3.0 to 17.1 +/- 1.4%, P less than 0.05. Serum levels of 1,25(OH)2D fell from 26.8 +/- 9.1 to 4.5 +/- 5.5 pg/ml after 17 days of Al; serum 25(OH)D levels were unchanged. These data indicate that Al can cause OM and that its severity correlates with the bone Al content.2 +
骨铝（Al）蓄积与透析相关性骨软化症（OM）之间存在关联。为研究铝在骨软化症中是否具有致病性，对6只犬在静脉注射铝（1毫克铝/千克/天）3至5周之前（Bx 1）和之后（Bx 2）进行了定量骨组织形态计量学研究。通过组织化学和化学方法测定骨铝含量。铝处理后，类骨质百分比从2.8±0.8%升至7.0±4.3%（平均值±标准差），P<0.05，类骨质宽度从5.7±0.6微米增加到8.0±1.2微米，P<0.01。铝处理后，骨铝含量从1.3±1.6毫克/千克升至94.0±19.0毫克/千克，以成骨表面百分比或类骨质百分比表示的骨软化症严重程度，与通过组织化学测量并以铝染色的小梁骨表面百分比或面积百分比表示的骨铝含量相关（r = 0.85 - 0.90，P<0.01）。在Bx 2时发现四环素摄取不良（6只犬），这表明矿化受损，并且四环素标记几乎没有或没有分离（4只犬）；因此，骨沉积和形成率低于检测限。吸收表面没有变化，但以组织体积百分比表示的小梁体积从22.1±3.0%降至17.1±1.4%，P<0.05。铝处理17天后，血清1,25(OH)2D水平从26.8±9.1皮克/毫升降至4.5±5.5皮克/毫升；血清25(OH)D水平未改变。这些数据表明铝可导致骨软化症，其严重程度与骨铝含量相关。
