Griffith R C, Scharp D W, Hartman B K, Ballinger W F, Lacy P E
Diabetes. 1977 Mar;26(3):201-14. doi: 10.2337/diab.26.3.201.
Isologous pancreatic islets were implanted into the portal vein of rats with streptozotocin-induced diabetes. At intervals of from one to 32 days after transplantation, the intrahepatic islet grafts were examined histologically and ultrastructurally, and their vascular supply was determined by later perfusion studies. Implanted islets were found widely dispersed throughout the liver in peripheral interlobular portal venules and surrounded by vacuolated liver cells containing large stores of glycogen. The endocrine cells were structurally normal in each interval examined. By the third day after transplantation the beta cells were depleted of secretory granules in aldehyde-fuchsin preparations. Regranulation returned by the 14th day and was associated with secretory organelle hypertrophy and hyperplasia. Islet cells were found outside the portal areas in direct apposition to hepatocytes forming distinct desmosomes by the first day. While hemoperfusion of the grafts occurred from the moment of implantation into the portal venule, a dual vascular supply derived from periportal arterial and venous sources developed by the 11th day after transplantation, establishing full vascularization of the grafts. Preliminary work is presented to show that an active ingrowth of nerves in the islet graft occurs in association with the process of vascularization.
将同种异体胰岛植入链脲佐菌素诱导的糖尿病大鼠的门静脉。在移植后1至32天的不同时间间隔，对肝内胰岛移植物进行组织学和超微结构检查，并通过后期灌注研究确定其血管供应。发现植入的胰岛广泛分散在肝脏外周小叶间门静脉小静脉中，并被含有大量糖原的空泡化肝细胞包围。在所检查的每个时间间隔内，内分泌细胞结构正常。移植后第三天，醛复红制剂中的β细胞分泌颗粒减少。到第14天重新出现颗粒，且与分泌细胞器肥大和增生有关。移植第一天就发现胰岛细胞在门静脉区域外直接与肝细胞相邻，形成明显的桥粒。虽然从植入门静脉小静脉的那一刻起移植物就发生了血液灌注，但移植后第11天形成了来自门静脉周围动脉和静脉来源的双重血管供应，实现了移植物的完全血管化。初步研究表明，胰岛移植物中神经的活跃长入与血管化过程相关。
